Evaluation of vacuolar ATPase and identification of salt stress responsive genes in cotton

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dc.contributor.advisorSangha, Manjeet Kaur
dc.contributor.authorCharandeep Singh
dc.date.accessioned2019-07-09T10:52:26Z
dc.date.available2019-07-09T10:52:26Z
dc.date.issued2019
dc.description.abstractThe present study evaluated the effect of salinity stress on various ATPase activities i.e vacuolar (V)-ATPase, F-ATPase and plasma (P)-ATPase in salt tolerant (LH 2108 (Gossypium hirsutum) and LD 949 (G.arboreum)) and salt susceptible (F1378, F2228 (G. hirsutum) and FDK124 (G.arboreum)) cotton genotypes. The seeds were grown in pots at salinity levels of 9, 15 and 18 dS/m. The leaf and root samples were collected at 15 and 30 days after germination. In response to salinity the V-ATPase activity registered maximum increase followed by F-ATPase, while P-ATPase registered decline. LH2108 (G. hirsutum) showed maximum increase in V-ATPase and F ATPase activities followed by that in LD 949 (G.arboreum). LH 2108 (G.hirsutum), registered 27.05 to 35.26μmol Pi formed h-1 mg-1 protein increase in mean leaf V-ATPase activity followed by that in LD 949 (G. arboreum) (27.30 to 34.25 μmol Pi formed h-1 mg-1 protein) from control to 18EC. Similar trend was observed for root samples and for protein content in leaf and root tissues. Genes (19) of salinity tolerance pathways viz. SOS4, HKT, HDG, GhPLATZ, SCARECROW, ABR1, GbRLK1, WRKY33, lysophospholipase, β-ketoacyl CoA synthase, ROS scavenging activity including GhSOD1, udp glucosyltransferase, GhMT3a, GhAnn1, ZAT10, WRKY39-1, osmolytic regulation P5CS, sucrose phosphate synthase, sorbitol dehydrogenase and trehalose synthase, selected by in silico analysis, showed their presence (by PCR amplification) in the selected (19) cotton genotypes. The salt tolerant G.hirsutum genotype showed better salinity tolerance which might be due to higher V and F-ATPase activities and also might be due to the presence of the selective salinity stress responsive genes.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810113492
dc.keywordsATPase, cotton, genes, salinity, toleranten_US
dc.language.isoenen_US
dc.pages61en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemEvaluation of vacuolar ATPase and identification of salt stress responsive genes in cottonen_US
dc.subBiochemistryen_US
dc.subjectnullen_US
dc.themeEvaluation of vacuolar ATPase and identification of salt stress responsive genes in cottonen_US
dc.these.typeM.Scen_US
dc.titleEvaluation of vacuolar ATPase and identification of salt stress responsive genes in cottonen_US
dc.typeThesisen_US
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