MICROSATELLITE MARKER BASED CHARACTERIZATION OF INDIGENOUS PIGS OF KERALA
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Date
2006
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College of Veterinary and Animal Sciences, Mannuthy.
Abstract
The study was undertaken to assess the genetic diversity among four
indigenous pig population of Kerala using microsatellite markers. The animals
were selected from various part of Kerala, population I included the conserved
Angamali pigs from university farm, Mannuthy, population II consisted of animals
from Koothattukualm, population III were the animals from Ollur and animals from
border districts of Kerala formed the population IV. Genetic analysis was carried
out using five polymorphic microsatellite markers.
Blood samples were collected from 100 unrelated indigenous pigs from all
four populations and DNA was isolated. The phenol-chloroform method of
extraction yielded 224.35±9.86pg/5ml of blood. PGR conditions were standardized
for all five selected markers namely, S0005, SOlOl, SW1026, SW2517 and S0008.
The forward primer of each marker was endlabelled with 732 P-ATP as source of
radio signal. The Ml3 single strand DNA was sequenced and used as a size
standard.
Autoradiography was employed to visualize the results. A total of eight
alleles were detected in S0005 and SOlOl, five alleles in each of SW1026 and
S0008, and six in SW2517. The heterozygosity varied from 0.7747 in SW2517 to
as large as 0.8475 for S0005. The heterozygosity values for SOIOI, SW1026 and
S0008 were 0.7774, 0.7672, and 0.7424 respectively. The PIC values ranges from
0.6974 for S0008 to 0.8291 for S0005. The PIC values for SOIOI, SW1026 and
SW2517 were 0.7483, 0.7284 and 0.7381 respectively.
The allele frequencies were used to estimate the Nei's standard genetic
distance among the populations. The distance measure ranged from 0.5704 to
0.7161, with the highest value noticed between population II and IV and the lowest
between population I and III.
A dendrogram was constructed using the POPGENE version 3.2 program
which grouped the population I and IV in one cluster and II and III populations in
another cluster.
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