Molecular characterization of multicut forage sorghum [Sorghum bicolor (L.) moench] using SSR markers

Simple item page
dc.contributor.advisorBoora, K.S.
dc.contributor.authorYadav, Anita
dc.date.accessioned2016-10-29T08:43:04Z
dc.date.available2016-10-29T08:43:04Z
dc.date.issued2012
dc.description.abstractSorghum is a diverse genus belonging to the tribe Andropogoneae and family Poaceae. (2n=20). The present study was undertaken to analyze genetic variability among F2 progenies of a cross between HJ541 (single cut variety) and SSG59-3 (multicut variety) by using SSR markers. Fifty SSR primers were used to study genetic variability among 32 F2 progenies along with the parents. A total of 78 amplified DNA products were observed out of which 26 were monomorphic and 52 were polymorphic. Average percentage polymorphism across the 32 progenies was 46.2%. The number of amplified DNA bands varied from 2 to 7 with an average of 1.81 bands per primer. The size of amplified alleles ranged from 110- 1000 bp respectively. Analysis of data grouped the 32 progenies into two major clusters. Cluster I constitutes of 29 F2 progenies and the parent SSG 59-3 whereas Cluster II had the remaining F2 progenies and parent HJ 541. Genetic similarity based on ‘Simqual’ l sub programme among various progenies ranged from 0.45 to 0.81 indicating moderate genetic variability among F2 population along with their parents. The average similarity between 32 progenies was found to be 0.66. Parental varieties were found to be most diverse followed by F2 progenies G18 and G7 with a coefficient of 0.45. F2 progeny G12 and G13, G9 and G11, G9 and G30, G29 and G30 were most similar with 0.81 coefficient of similarity. Two and three dimensional principle component analysis (PCA) also gives similar clustering of 32 F2 progenies. The highly diverse F2 progenies may be used for breeding of multicut varieties of sorghum in order to meet ever increasing demand of green fodder for the livestock. SSR would provide adequate coverage of genome for germplasm identification and pedigree validation.en_US
dc.description.abstractSorghum is a diverse genus belonging to the tribe Andropogoneae and family Poaceae. (2n=20). The present study was undertaken to analyze genetic variability among F2 progenies of a cross between HJ541 (single cut variety) and SSG59-3 (multicut variety) by using SSR markers. Fifty SSR primers were used to study genetic variability among 32 F2 progenies along with the parents. A total of 78 amplified DNA products were observed out of which 26 were monomorphic and 52 were polymorphic. Average percentage polymorphism across the 32 progenies was 46.2%. The number of amplified DNA bands varied from 2 to 7 with an average of 1.81 bands per primer. The size of amplified alleles ranged from 110- 1000 bp respectively. Analysis of data grouped the 32 progenies into two major clusters. Cluster I constitutes of 29 F2 progenies and the parent SSG 59-3 whereas Cluster II had the remaining F2 progenies and parent HJ 541. Genetic similarity based on ‘Simqual’ l sub programme among various progenies ranged from 0.45 to 0.81 indicating moderate genetic variability among F2 population along with their parents. The average similarity between 32 progenies was found to be 0.66. Parental varieties were found to be most diverse followed by F2 progenies G18 and G7 with a coefficient of 0.45. F2 progeny G12 and G13, G9 and G11, G9 and G30, G29 and G30 were most similar with 0.81 coefficient of similarity. Two and three dimensional principle component analysis (PCA) also gives similar clustering of 32 F2 progenies. The highly diverse F2 progenies may be used for breeding of multicut varieties of sorghum in order to meet ever increasing demand of green fodder for the livestock. SSR would provide adequate coverage of genome for germplasm identification and pedigree validation.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/82871
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subMolecular Biology and Biotechnology
dc.subjectSorghum, Genetics, dna, Polymorphism, Genotypes, Alleles, Biological phenomena, Genetic structures, Locus, Cropsen_US
dc.subjectSorghum, Genetics, dna, Polymorphism, Genotypes, Alleles, Biological phenomena, Genetic structures, Locus, Cropsen_US
dc.these.typeM.Sc
dc.titleMolecular characterization of multicut forage sorghum [Sorghum bicolor (L.) moench] using SSR markersen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
CCSHAU-293057- Yadav, Anita.pdf
Size:
681.28 KB
Format:
Adobe Portable Document Format
Description:
Molecular characterization of multicut forage sorghum [Sorghum bicolor (L.) moench] using SSR markers
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
M. Sc. Dissertations