STUDIES ON IMMUNOMODULATORY AND GROWTH PROMOTING EFFECTS OF CLOVE OIL IN BROILER CHICKEN
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Date
2020
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Department of Veterinary Pharmacology and Toxicology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand
Abstract
To assess immunomodulatory activity, a total of 60 chicks were divided randomly to 5 groups each of 12 chicks. Group I served as control and given basal diet without clove oil and vitamin E and selenium. Group II served as standard control and given vitamin E and selenium containing proprietary product in water at the dose rate of 1.5 grams per 100 birds for first two weeks and 5 grams per 100 birds for next 3 weeks. The remaining groups III, IV and V were given clove oil at the dose rate of 200, 400 and 800 mg/kg diet, respectively. This study was conducted for 35 days. Cutaneous basophil hypersensitivity (CBH) response at two different doses (100 μg and 200 μg) of phytohemagglutinin-P (PHA-P) was carried out to assess cell mediated immunity on 14th day of age. Blood was collected on 7th, 21st and 35th day of age and serum was separated to estimate antibody titers against Newcastle Disease Virus vaccine by haemagglutination inhibition (HI) test and biochemical parameters like serum total protein, serum albumin, serum globulin and albumin to globulin ratio (A/G). On 35th day, thin blood smears were prepared and stained with field’s stain to determine differential leucocyte counts microscopically. At the end of the experiment, birds of all groups were sacrificed and tissues like thymus, spleen and bursa were collected for histopathological examinations.