STUDIES ON IN VITRO CLONAL PROPAGATION OF ROSE (Rosa damascena Mill.)
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Date
1993
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AAU, Anand
Abstract
THe present study was undertaken to develop a protocol for a raicropropagation of rose, Rosa damascena Mill, by standardizing proper time of collection of explant, identifying suitable media for obtaining sprouting, maximum multiplication rate and rooting response. The results of the study showed that the maiximum number of sprouting/bud breakage were obtained when explants were collected 3 days after decapitation and 4th nodal position from the top. The Murashige and Skoog's (1962) media supplemented with 0.25 mgl-1 gibberellic acid (GA ) and 0.5 mgl-1 benzyl adenine (BA) was found to be the best for establishment and sprouting of the bud. Nodes from the middle portion of the branch recorded better sprouting and vigorous growth as compared to that from the top and bottom. The maximum proliferation/multiplication rate could be achieved by using MS media incorporated with 0.5 mgl-1 BA and 0.35 mgl-1 GA . It was noted that BA is very important for the multiplication of the shoots. Repeated subculturing in the same media upto 3 subculture have shown good multiplication rate thereafter the rate was reduced. The effect of different strengths of MS media supplemented with 0.25 mgl-1 NAA and 0.1 mgl-1 2,4-D on rooting after keeping for different durations in induction media were studied. The 1/4 strength of media was most suitable for good rooting when shoots were kept for three weeks, in induction media. Different potting mixtures were tried to obt«iin maximum survival of in vitro raised plants. The rooted plaikts were transferred into the pot for gradual hardening. The highest survival rate of 90% was found in pot mixtures of sand, soil, FYM and vermiculite ( 1 : 1 : 1 : 1 ) .
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PLANT PHYSIOLOGY AND ECOLOGY, A STUDY