Molecular characterization of pseudocercospora species causing sigatoka disease complex in banana (musa spp.)
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Date
2021-08-31
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Banana (Musa spp.) is an important horticultural crop in the World. It is themostdeliciousfruitconsumedastablepurposeaswellasculinaryfruit.However,duetonarrow genetic pool, banana plants are more prone to various diseases. The diseases,Sigatoka disease caused by Pseudocercospora species is threatening banana plantationsatMaharashtraandotherbanana growingstatesof India.Studiesareconductedwiththeaim to identify genetic diversity among various member species of Pseudocercosporaacross various geographical locations. Among 36 isolates collected from different statesof India, 13 isolates were confirmed as members of P. musicola species using species-specific primer. The cultural and morphological characterization study showed widevariations with respect to mycelial colour, pigmentation, and growth pattern. Based onmorphological characterization, 13 isolates were categorized into three distinct groupsnamely fast, moderate and slow growing isolates. During molecular characterization, 29(RAPD)randomprimersand18ISSRprimerswerescreenedand,amongwhich5RAPDand6ISSRprimershavegeneratedreproducibleandscorablebandingpatternrespectively. The RAPD andISSR marker data analysis represented 67% to 87%.Genetic similarity among 13 members of P. musicola. The cluster analysis based onsimilarity matrix data could categorize 13 isolates into two major groups. Study isconcluded with the presence of high degrees of genetically variable population withinmember species P. musicola and two-way marker system (RAPD+ISSR) was foundeffectiveindiscriminatingthememberpopulationofP.musicolaspeciescollectedfrombanana growingregion ofIndia.