Genetic diversity and domestication of Pyrenacantha volubilis Wight, an anti cancer drug yielding plant
| dc.contributor.advisor | Santhoshkumar, A V | |
| dc.contributor.author | Arjun Ramachandran | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2021-08-25T07:07:27Z | |
| dc.date.available | 2021-08-25T07:07:27Z | |
| dc.date.issued | 2020 | |
| dc.description | PhD | en_US |
| dc.description.abstract | The present study titled “Genetic diversity and domestication of Pyrenacantha volubilis Wight: an anti-cancer drug yielding plant” was carried out from 2017 to 2020 with the objectives of exploring the genetic diversity in fragmented natural populations of P. volubilis, analyzing camptothecin and its major derivatives isolated from P. volubilis for druggability against major oncoproteins, identifying elite lianas through a progeny trial and propagating the plant vegetatively. UPGMA classified the 12 natural populations of P. volubilis into seven distinct classes based on the amplicon profiles of the 12 ISSR primers considered for the study. There is high genetic diversity among populations of P. volubilis, the full potential of which must be tapped for domestication. The binding energy computed for interactions of camptothecinoids with oncoproteins and Topo I were unstable because camptothecinoids act on the ‘cleavable complex’. 20 three-year-old lianas (PV01 to PV20) superior for vegetative and reproductive growth traits were screened by progeny evaluation. CPT accumulation in vegetative parts was in trace amounts for all the nine progenies tested in this experiment. The maximum harvest weight of seeds per plant was for progeny PV10-01 (4.57g). The concentration of camptothecin (2.12%) coupled with prolific seed bearing (9 seeds per plant) after the first year itself makes PV10-01 superior to all other progenies for overall camptothecin yield. Results of the progeny trial indicate that mother plants PV01, PV02, PV06, PV08 and PV10 may be considered for higher economic gains. Four to eight noded stem cuttings (softwood, semi-hardwood, hardwood) were treated with IBA (0, 2000, 4000, 6000, 8000 and 10000mgL-1) and laid out in a 3×6 Factorial CRD in a semi-automatic mist chamber to standardize vegetative propagation. Hardwood cuttings treated with 2000mgL-1 (83.33%) and 4000mgL-1 (75.56%) IBA and softwood cuttings treated with 4000mgL-1 (60.12%) IBA were superior for rooting percentage. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810174394 | |
| dc.keywords | Forest Biology, Tree Improvement, anti cancer drug yielding plant | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 106p. | en_US |
| dc.publisher | Department of Forest Biology and Tree Improvement, College of Forestry, Vellanikkara | en_US |
| dc.sub | Forest Biology and Tree Improvement | en_US |
| dc.theme | Genetic diversity of Pyrenacantha volubilis Wight | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Genetic diversity and domestication of Pyrenacantha volubilis Wight, an anti cancer drug yielding plant | en_US |
| dc.type | Thesis | en_US |