DIALLEL ANALYSIS IN INTERSPECIFIC LINES OF MUSTARD (Brassica spp.) AND IDENTIFICATION OF MOLECULAR MARKERS LINKED TO APHID RESISTANCE
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Date
2019
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DEPARTMENT OF GENETICS AND PLANT BREEDING B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
The present investigation entitled “Diallel analysis in interspecific lines of
mustard (Brassica spp.) and identification of molecular markers linked to aphid
resistance” was undertaken to generate information on the magnitude of heterosis,
gene action and combining ability effects of parents and F1s for quantitative and
qualitative traits in order to suggest a sound breeding methodology for development
of high yielding genotypes/ hybrids. The other purpose of this study was to identify
aphid resistance linked markers. PCR-based molecular approaches like SSR, CAPS
and RAPD markers were used to attain the objective. The research work was carried
out at farm of “Research Centre for Distant Hybridization in Field and Fruit Crops”
and Biotechnology laboratory, Centre of Excellence in Biotechnology, Dept. of
Agricultural Biotechnology, A.A.U., Anand. The crosses were made between
interspecific lines taken as parents in rabi 2016-17 whereas the evaluation of parents
and hybrids was done in consecutive rabi 2017-18. The screening of 200 F8 RILs for
aphid resistance was conducted in rabi 2018-19.