DNA FINGERPRINTING AND DIVERSITY ANALYSIS OF NERIUM (Nerium oleander L.) CULTIVARS AND HYBRIDS

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dc.contributor.advisorP. PAVAN KUMAR
dc.contributor.advisorSHIROL, A. M.
dc.contributor.advisorGUNNAIAH, RAGHAVENDRA.
dc.contributor.advisorJHALEGAR, Md. JAMEEL.
dc.contributor.advisorMETI, SHANKAR.
dc.contributor.advisorPATIL, BAPURAYAGOUDA.
dc.contributor.authorMELAVANKI, PARASHURAM.
dc.date.accessioned2023-11-04T12:34:56Z
dc.date.available2023-11-04T12:34:56Z
dc.date.issued2021-01
dc.description.abstractAn experiment entitled ‘DNA fingerprinting and diversity analysis of Nerium (Nerium oleander L.) cultivars and hybrids’ was conducted at Department of Floriculture and Landscape Architecture, College of Horticulture, Bagalkot, University of Horticultural Sciences, Bagalkot during the 2018 to 2020. Evaluation of nerium cultivars for growth and qualitative traits, floral biology, in vivo pollen germination and pollen pistil interaction were investigated. Fourteen direct intervarietal cross combinations were conducted, fruit setting and seed germination behaviour of different cross combinations were studied. In vitro inter varietal immature seed culture was studied. The DNA fingerprinting as well as diversity analysis of nerium cultivars and hybridity conformation at molecular level were investigated. The results revealed that maximum value for plant growth increment UHSBN10 (226.20 cm), plant spread in East-West UHSBN-29 (199.79 cm), plant spread in North-South UHSBN-13 (201.47 cm), number of primary branches UHSBN-27 (6.03), number of secondary branches UHSBN-1 (13.28), inter nodal length UHSBN-29 (5.40 cm), leaf length UHSBN-29 (23.50 cm), leaf width UHSBN-12 (3.02 cm) and petiole length UHSBN-21 (1.16 cm) were recorded in the nerium cultivars. Maximum mean number of inflorescence per plant UHSBN-12 (27.92), number of flowers per inflorescence UHSBN-2 (90.23), bud length UHSBN-13 (3.28 cm), corolla tube length UHSBN-13 (1.22 cm), flower diameter UHSBN-18 (5.86 cm), individual flower weight UHSBN- 27 (1.40 g), hundred flower weight UHSBN-27 (139.90 g) and yield per plant UHSBN-17 (4.92 kg) were recorded in the prasent investigation. Anthesis, anther dehiscence and stigma receptivity were observed during the morning hours. Irrespective of nerium cultivars, pollen viability (88.77 to 97.90 %) and quality of the pollen grains (61.20 to 77.12 %) was good, it is suggesting that all the studied cultivars can be used as male parent in hybridization programme. Observation of stigmatic papillae under florescent microscope reveals that presence of herkogamy in the nerium. Receptive area on the stigmatic papillae was restricted in nerium which was present at below the stylar ribs on both the sides. In vivo pollen germination and pollen 304 pistil interaction by selfing in popular cultivar UHSBN-12 indicates that no barriers were present. Nerium is self-compatible, but automatic selfing was prevented by the presence of sticky drop. Most of the pollen tube showing irregular growth, twisting and branching of pollen tubes were noticed in Double X Double type cultivar crosses. There was wide variation with respect to pod set was observed and it ranged from 12 to 87.27 % among the different cross combinations. The cross combination UHSBN-14 X UHSBN -3 was recorded the maximum seed viability per cent (96.66) and maximum seed germination were noticed in the cross combination UHSBN-12 X UHSBN-14 (66.00 %). By following the immature in vitro intervarietal seed culture it is possible to reduce the time up to 6 months for one hybridization cycle when compare to traditional breeding cycle. Fifteen ISSR primers were used to produce DNA fingerprint profiles of nerium cultivars. Among the fifteen primers used for the study, eleven primers showed polymorphism with the 122 loci across thirty neruim cultivars. Out of these loci, 111 loci were polymorphic (89.98 %), reflecting the richallelic diversity in the sampled populations. Thirty nerium cultivars were clustered into two major clusters by the Mega X based on the polymorphic bands with total branch length of 382.09. Major cluster-I consisting of 23 cultivars with total sub tree distance of 272.17 and cluster II consisting of 7 cultivars with sub tree distance of 109.92. ISSR analysis was carried out in five randomly selected individuals of nerium F1 hybrids in each cross combination, which exhibited intermediate characteristics to confirm hybridity on the molecular level. Band pattern was evaluated and male parent informative bands were present in the sample of the progeny, were verified as hybrids.
dc.identifier.otherUHS17PGD205
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810200375
dc.keywordsHorticulture
dc.keywordsFloriculture
dc.keywordsLandscape Architecture
dc.keywordsDNA Fingerprinting
dc.keywordsNerium
dc.keywordsCultivars
dc.keywordsHybrids
dc.keywordsTraits
dc.keywordsFloral Biology
dc.keywordsIn Vivo
dc.keywordsPollen
dc.keywordsGermination
dc.keywordsPollen Pistil
dc.keywordsIn Vitro
dc.keywordsMolecular
dc.keywordsInter Nodal Length
dc.keywordsPetiole Length
dc.keywordsInflorescence
dc.keywordsCorolla Tube Length
dc.keywordsAnthesis
dc.keywordsAnther Dehiscence
dc.keywordsStigma
dc.keywordsHybridization
dc.keywordsPollen Grains
dc.keywordsFlorescent
dc.keywordsHerkogamy
dc.keywordsPapillae
dc.keywordsRichallelic
dc.keywordsPolymorphic
dc.keywordsUHS17PGD205
dc.keywordsT701
dc.language.isoEnglish
dc.pages324p. (Due to pdf. conversion there is a difference in pages of soft copy and hard copy).
dc.publisherUniversity of Horticultural Sciences, Bagalkot. (College of Horticulture, Bagalkot).
dc.subFloriculture and Landscaping
dc.themeDNA FINGERPRINTING AND DIVERSITY ANALYSIS OF NERIUM (Nerium oleander L.) CULTIVARS AND HYBRIDS
dc.these.typePh.D
dc.titleDNA FINGERPRINTING AND DIVERSITY ANALYSIS OF NERIUM (Nerium oleander L.) CULTIVARS AND HYBRIDS
dc.typeThesis
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