Fine mapping of male-sterility ms-1 gene using whole genome resequencing of muskmelon (Cucumis melo L.)

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dc.contributor.advisorSarao, Navraj Kaur
dc.contributor.authorSimranjot Kaur
dc.date.accessioned2022-02-24T10:43:27Z
dc.date.available2022-02-24T10:43:27Z
dc.date.issued2021
dc.description.abstractMuskmelon (Cucumis melo L.) is a warm season crop, cultivated in tropical and temperate regions of the world. Five types of male-sterility genes have been reported in this crop, out of which ms-1 and ms-5 has been utilized in hybrid seed production programmes. The identification of molecular markers linked to these male-sterile genes may find practical applications in hybrid seed production and marker-assisted backcross (MABC) programmes. The previously found SSR markers linked to ms-1 gene are quite at a distance to be considered as tightly-linked. Therefore, the current study was aimed at fine mapping the genomic loci harbouring the ms-1 gene using whole genome resequencing approach. A cross between male-sterile MS-1 and male-fertile KP4HM-15 was used to raise an F2 mapping population for the construction of two contrasting bulks namely, male-sterile bulk (MSB) and male-fertile bulk (MFB). These bulks along with both the parents were sequenced and analyzed using SNP-index method for identifying the ms-1 associated region. A 400-kb candidate region on chromosome six was identified using this method which lied within the previously mapped 3.2 Mb region using SSR markers. This candidate region harboured nine highly-significant SNPs and 28 annotated genes. The nine highly-significant SNPs can be used to develop KASP markers for the further improvement of genetic map. It was observed that out of 28 annotated genes, six genes were involved in imparting male-sterility in other crops and therefore these six genes were regarded as candidate putative genes. It was also noticed that the genes fasciclin-like arabinogalactan protein 1 (FLA) and ABC transporter G family member 26, each harboured a highly-significant SNP. Also, one of gene named protein-disulfide isomerase (PDI) contained a missense variation from „A‟ to „T‟ at 36,921,314 bp position. This missense mutation in the PDI gene might be responsible for the male-sterility in ms-1 plants.en_US
dc.identifier.citationSimranjot Kaur (2021). Fine mapping of male-sterility ms-1 gene using whole genome resequencing of muskmelon (Cucumis melo L.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182833
dc.keywordsWhole genome resequencing, ms-1 gene, muskmelon, BSAen_US
dc.language.isoEnglishen_US
dc.pages77en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemFine mapping of male-sterility ms-1 gene using whole genome resequencing of muskmelon (Cucumis melo L.)en_US
dc.subAgricultural Biotechnologyen_US
dc.themeFine mapping of male-sterility ms-1 gene using whole genome resequencing of muskmelon (Cucumis melo L.)en_US
dc.these.typeM.Scen_US
dc.titleFine mapping of male-sterility ms-1 gene using whole genome resequencing of muskmelon (Cucumis melo L.)en_US
dc.typeThesisen_US
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