DETECTION OF ANTIGENIC VARIANTS OF CANINE PARVOVIRUS BY POLYMERASE CHAIN REACTION AND SEQUENCING
| dc.contributor.advisor | M. Mini | |
| dc.contributor.author | MEENU BASHEER | |
| dc.date.accessioned | 2020-07-10T06:02:59Z | |
| dc.date.available | 2020-07-10T06:02:59Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | A study was conducted to detect the antigenic variants of canine parvovirus from faecal samples of clinically suspected dogs by PCR and sequencing. The samples were screened for the presence of CPV by HA test and the results were confirmed by HI test. Antigenic characterisation of field strains and vaccine strains was performed by employing PCR with differential pairs of primers and PCR-RFLP analysis. The partial sequences obtained from the amplified CPV VP2 gene fragment of nine samples were analysed to identify the nucleotide variations. A total of 78 faecal samples were collected from dogs suspected for CPV infection that were brought to veterinary hospitals attached to KAU and District Veterinary Centre, Thiruvananthapuram. Twenty eight (35.90 per cent) out of 78 samples were found to be positive to CPV infection by HA test and the results were further confirmed by HI test. Among 78 faecal samples tested by PCR, none of the samples were found positive for CPV-2, while 57 (73.08 per cent) were found positive with CPV-2ab primers. Out of 57 CPV-2ab positive samples, 15 were identified as CPV-2a types while remaining 42 were CPV-2b types. The specificity of PCR was further confirmed by RE digestion using Hha I enzyme. Thus, CPV-2b was identified as the predominant CPV type in this study. Among the four vaccines tested, Megavac-6, Nobivac® Puppy DP and Vanguard® 5 vaccines were found to be classical CPV-2 based vaccines while Duramune® max vaccine was found to contain the CPV-2b variant. All the 57 CPV-2ab positive samples, when subjected to PCR with CPV555 primer set, yielded a specific amplicon of 583 bp. On PCR-RFLP analysis with Mbo II enzyme, none of these products showed specific CPV-2c cleavage pattern of 500 and 83 bp. This indicated the absence of CPV-2c in this area. Sequence analysis of the CPV VP2 gene from the PCR products of nine samples revealed that seven were CPV-2b types and the remaining two were “new CPV-2a” types. No CPV-2c types were detected among the sequenced samples. Additional mutations at nucleotide 4104 of residue 440 and at nucleotide 4121 of residue 445 were also observed in the CPV sequences under study. Sequence similarity searches of the CPV sequences using BLAST analysis revealed that the sequences were highly specific to CPV, as indicated by the maximum identity (99-100 per cent) obtained with VP2 gene sequences of other CPV strains available in the GenBank. Phylogenetic analysis revealed that most of the CPV samples under study were related to variants typical to the country. Hence the Indian isolates were found to be genetically close to Asian isolates suggesting the migration of variants crossing the borders of different countries. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810148953 | |
| dc.keywords | DETECTION ANTIGENIC VARIANTS CANINE PARVOVIRUS BY POLYMERASE CHAIN REACTION SEQUENCING | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 157 | en_US |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR | en_US |
| dc.research.problem | DETECTION OF ANTIGENIC VARIANTS OF CANINE PARVOVIRUS BY POLYMERASE CHAIN REACTION AND SEQUENCING | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | DETECTION OF ANTIGENIC VARIANTS OF CANINE PARVOVIRUS BY POLYMERASE CHAIN REACTION AND SEQUENCING | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | DETECTION OF ANTIGENIC VARIANTS OF CANINE PARVOVIRUS BY POLYMERASE CHAIN REACTION AND SEQUENCING | en_US |
| dc.type | Thesis | en_US |