Effect of priming with Moringa oleifera leaf extract on sorghum under salt stress

dc.contributor.advisorOberoi, Harpreet Kaur
dc.contributor.authorAnand Kumar
dc.date.accessioned2024-01-03T10:38:51Z
dc.date.available2024-01-03T10:38:51Z
dc.date.issued2022
dc.description.abstractIn the present study, seeds of fourteen sweet sorghum genotypes were treated with different NaCl concentrations (0, 50, 100, 150, 200 mM). All germination traits decreased with increased salinity. Based on germination traits and dendogram obtained at 100 mM NaCl, PHULE VASUNDHARA and SPV 2074 were selected as tolerant and susceptible genotypes, respectively. Seeds of selected genotypes were primed with 10% Moringa oleifera leaf extract and grown in Hoagland‟s solution with and without 100 mM NaCl concentration. NaClinduced the activities of SOD (in roots), POX and PPO and reduced the activities of NR, NiR and GOGAT, however, significant improvement in the activities were observed with MLE in shoots and roots of two contrasting genotypes at 10 DAG. Salt stress resulted in increased Na+/K+ ratio, H2O2, MDA, sugars, phenols and decreased total chlorophyll. Gene expression of antioxidative enzymes was upregulated under salt stress in shoots and roots of both primed genotypes. Salt stress resulted higher PPO, GOGAT, NiR and NR gene expressions in shoots of both contrasting genotypes with priming conditions. Expression of HAK gene was higher in primed SPV 2074 shoot and root without salt stress. In both tissues of tolerant and susceptible genotypes, expression of HKT-6 was higher with and without priming under both control and salt stress conditions. Therefore, the present investigation suggests that moringa seed priming impact the growth, enzymatic activities as well as gene expression under salt stress in sweet sorghum.en_US
dc.identifier.citationAnand Kumar (2022). Effect of priming with Moringa oleifera leaf extract on sorghum under salt stress (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810205441
dc.keywordsSalt stress, Sweet Sorghum, Germination, Antioxidant and Nitrogen metabolizing enzymes, Gene expression.en_US
dc.language.isoEnglishen_US
dc.pages104en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemEffect of priming with Moringa oleifera leaf extract on sorghum under salt stressen_US
dc.subBiochemistryen_US
dc.themeEffect of priming with Moringa oleifera leaf extract on sorghum under salt stressen_US
dc.these.typeM.Scen_US
dc.titleEffect of priming with Moringa oleifera leaf extract on sorghum under salt stressen_US
dc.typeThesisen_US
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