MODULATION OF PROSTAGLANDIN E2 (PGE2) MEDIATED PATHWAY IN THE ECLOSION BLOCKING EFFECT OF TERPENOID FRACTION OF ARTEMISIA NILAGIRICA IN RHIPICEPHALUS (BOOPHILUS) ANNULATUS

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dc.contributor.advisorSanis Juliet
dc.contributor.authorPANICKER DEVYANI RAMACHANDRAN
dc.date.accessioned2020-07-23T08:36:05Z
dc.date.available2020-07-23T08:36:05Z
dc.date.issued2018
dc.description.abstractProstaglandins are a group of important cell signaling molecules involved in the the regulation of ovarian maturation, oocyte development, egg laying and associated behaviours in invertebrates. However, the presence of prostaglandin E2 (PGE2), the key enzymes for biosynthesis and its interference by drugs have not been investigated in the ovary of ticks. The present study was undertaken to assess the modulation of PGE2 mediated pathway in the eclosion blocking effect of terpenoid fraction isolated from Artemisia nilagirica in Rhipicephalus (Boophilus) annulatus ticks. The dried hexane fraction of ethanolic extract of aerial parts of A. nilagirica was used for isolation of terpenoid fraction by Herz-Hogenauer method. The acaricidal activity of the terpenoid fraction was evaluated by adult immersion test. The chemical profiling of the extract/fraction was done by HPTLC and GCMS analysis. Localization of the cyclooxygenase1 and prostaglandin E synthase enzymes in the ovaries of control and treated ticks were done by immunohistochemistry. The vitellogenin concentration in hemolymph also was assayed by indirect ELISA. The terpenoid fraction of A.nilagirica elicited a concentration dependent percentage inhibition of fecundity and hatching rate in the laid eggs of R (B.) annulatus. The HPTLC fingerprint profiling of terpenoid fraction revealed the presence of nine polyvalent compounds. Twenty two terpenoid compounds were identified in the terpenoid fraction based on mass spectral matching with commercial libraries (NIST, Wiley). Presence of PGES in stage III oocytes in control ticks was confirmed by immunohistochemistry whereas immunereactivities were absent in the vitellogenic oocytes of flumethrin and terpenoid fraction treated ticks. The intensity of immunoreactivities were too low to be detected in vitellogenic oocytes of both control and treated ticks. The levels of PGE2 were below detection limit in in the ovaries of flumethrin treated ticks. The levels were significantly lower in ovaries of terpenoid fraction than control ovaries. No significant difference in the concentration of Vg was observed in the hemolymph of treated and control ticks.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149710
dc.keywordsMODULATION PROSTAGLANDIN E2 (PGE2) MEDIATED PATHWAY ECLOSION BLOCKING EFFECT TERPENOID FRACTION ARTEMISIA NILAGIRICA IN RHIPICEPHALUS (BOOPHILUS) ANNULATUSen_US
dc.language.isoenen_US
dc.pages73en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemMODULATION OF PROSTAGLANDIN E2 (PGE2) MEDIATED PATHWAY IN THE ECLOSION BLOCKING EFFECT OF TERPENOID FRACTION OF ARTEMISIA NILAGIRICA IN RHIPICEPHALUS (BOOPHILUS) ANNULATUSen_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.subjectnullen_US
dc.themeMODULATION OF PROSTAGLANDIN E2 (PGE2) MEDIATED PATHWAY IN THE ECLOSION BLOCKING EFFECT OF TERPENOID FRACTION OF ARTEMISIA NILAGIRICA IN RHIPICEPHALUS (BOOPHILUS) ANNULATUSen_US
dc.these.typeM.V.Sc.en_US
dc.titleMODULATION OF PROSTAGLANDIN E2 (PGE2) MEDIATED PATHWAY IN THE ECLOSION BLOCKING EFFECT OF TERPENOID FRACTION OF ARTEMISIA NILAGIRICA IN RHIPICEPHALUS (BOOPHILUS) ANNULATUSen_US
dc.typeThesisen_US
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MODULATION OF PROSTAGLANDIN E2 (PGE2) MEDIATED PATHWAY IN THE ECLOSION BLOCKING EFFECT OF TERPENOID FRACTION OF ARTEMISIA NILAGIRICA IN RHIPICEPHALUS (BOOPHILUS) ANNULATUS
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