OUTER MEMBRANE PROTEIN PROFILE ANALYSIS AND MULTILOCUS SEQUENCE TYPING OF PASTEURELLA MULTOCIDA ISOLATES OF BUFFALO ORIGIN FROM GUJARAT STATE
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Date
2015
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Publisher
AAU, Anand
Abstract
The present study was undertaken with a view to characterize Pasteurella
multocida isolates of buffalo origin (including vaccine strain P52) by outer membrane
protein (OMP) profiling and multilocus sequence typing (MLST). P. multocida
cultures of buffalo origin (suspected/died of Haemorrhagic Septicaemia) from various
regions of the state of Gujarat were stored in the Department of Veterinary
Microbiology, Veterinary College, Anand. All the isolates belonged to P. multocida
type B: 2 as determined by capsular typing conducted previously in the department. A
set of eight isolates including vaccine strain, P52 were used for OMP profile analysis,
while another set of eight isolates (including vaccine strain) were used in MLST.
Samples PAB-78-1/13, PAB-83-6/13, PAB-86-9/13 and PAB-89-1/14 were common
in both OMP and MLST analysis.
Description
Keywords
veterinary science, MICROBIOLOGY, ANALYSIS, SEQUENCE TYPING