COMPARATIVE EFFECT OF BACTERIOPHAGE AND NISIN ON Listeria monocytogenes IN CHICKEN AND FISH

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dc.contributor.advisorK Vrinda Menon
dc.contributor.authorSAJU GEORGE
dc.date.accessioned2020-07-24T09:31:34Z
dc.date.available2020-07-24T09:31:34Z
dc.date.issued2014
dc.description.abstractIn the present study, isolation of Listeria monocytogenes from chicken, fish and sewage was undertaken to understand its prevalence using standard culture method and confirmation by polymerase chain reaction (PCR). About 60 samples of chicken were collected and screened for the evaluation of the L. monocytogenes and none of the samples were found positive. Out of the 60 samples of fish collected from retail outlets from markets of Thrissur, Kozhikode and Palakkad, 5 samples were found positive for Listeria spp and out of which one isolate was confirmed as L.monocytogenes. The other four samples were confirmed as L.innocua. All positive samples were obtained from the municipal markets of Kozhikode. A total of 110 samples of sewage were screened for the presence of L. monocytogenes and three positive samples were obtained and the isolates were confirmed as L.innocua using PCR. The prevalence rate of Listeria in sewage was found to be 8.33 per cent. Sewage samples (110) were screened for the presence of Listeria specific bacteriophages and plaques could be isolated from 18 samples. The standardisation of PCR for the isolates was done using lysZ5gene. However, none of the isolated samples were found positive to this gene and was detected only in the standard culture (ATCC23074 B1). Comparative effect of bacteriophage and nisin on L.monocytogenes in chicken and fish were assessed. Bacteriophage (103 pfu/ml) and nisin (1 per cent) was added on the substrates at 10µl, 20µl and 30µl levels. Bacteriophage at 20µl level could completely inhibit the growth of L.monocytogenes on day 14 and day 7 of storage in chicken and fish respectively. But on addition of 30µl of nisin complete inhibition of bacteria was observed on 14th and 3rd day of storage in chicken and fish. However regrowth of bacteria was observed on further storage upto 21 days. Hence these antimicrobial substances have a great potential to control the growth of bacteria in foods. 86 Annexuresen_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149799
dc.keywordsCOMPARATIVE EFFECT BACTERIOPHAGE NISIN Listeria monocytogenes CHICKEN FISHen_US
dc.language.isoenen_US
dc.pages83en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSURen_US
dc.research.problemCOMPARATIVE EFFECT OF BACTERIOPHAGE AND NISIN ON Listeria monocytogenes IN CHICKEN AND FISHen_US
dc.subVeterinary Public Healthen_US
dc.subjectnullen_US
dc.themeCOMPARATIVE EFFECT OF BACTERIOPHAGE AND NISIN ON Listeria monocytogenes IN CHICKEN AND FISHen_US
dc.these.typeM.V.Sc.en_US
dc.titleCOMPARATIVE EFFECT OF BACTERIOPHAGE AND NISIN ON Listeria monocytogenes IN CHICKEN AND FISHen_US
dc.typeThesisen_US
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