Production of extracellular proteases from Pleurotus florida (Mont.) Sing. and Volvariella volvacea (Bul. Fr.) Sing.
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
Pleurotus florida and Volvariella volvacea are grown widely in subtropical and tropical areas.
Proteases are a group of fundamentally hydrolytic enzymes that acylate and deacylate to
peptide bonds. P.florida showed higher mycelial dry weight (13.4g/L) and wheat straw gave
maximum yield for P.florida (72.5kg/q). In qualitative estimation fruit body of V.volvacea
showed maximum halo diameter (1.17 cm). In quantitative analysis, for serine proteases in
crude and partially purified extract, mycelium of P. florida (28.1 nmol/min/mg) and fruit
body of P.florida (148.3 nmol/min/mg), showed maximum specific activity. The maximum
specific activity of cysteine proteases in crude and partially purified extract was observed in
fruit body of V.volvacea (28.1 nmol/min/mg) and fruit body of P.florida (145.2
nmol/min/mg). The maximum specific activity of aspartic proteases in crude and partially
purified extract was observed in mycelium of P.florida (42.2 nmol/min/mg) and fruit body of
P.florida (318.5 nmol/min/mg). Maximum molecular weight of protein was 50.2 kDa
recorded in all the samples. The FTIR (Fourier transform infrared spectroscopy) assay
indicated that the spent substrate of V.volvacea was devoid of Amide A bond, aromatic amino
acids and β-sheet conformation. Arginine, lysine and histidine were present in all the samples,
whereas sulfur containing amino acids were present in some of the samples. The proteases
production potential is more in P.florida than V.volvacea.
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