“Isolation and characterization of Aeromonas species from fish
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Date
2009
Authors
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Publisher
Anand Agricultural University, Anand
Abstract
The present study was undertaken with the objective to isolate and characterize
the Aeromonas spp. from raw fish intended for the human consumption available in
the retail fish market in Anand, Gujarat.
A total of 100 raw fish samples (muscles) were collected from the fish market.
Samples were processed with pre-enriched in alkaline peptone water (pH 8.4 ± 0.2) at
37oC for 24 h and further inoculated on three selective media viz., Ampicillin starch
DNA agar (SAA), Ampicillin dextrin agar (ADA) and Aeromonas isolation agar
(AIA). The presumptive isolates were identified at genus and species level by the
battery of biochemical tests and also confirmed by polymerase chain reaction. The
PCR confirmed isolates were further subjected for the antibiotic susceptibility test
using fourteen antibiotics and detection of virulence genes by PCR
Description
Keywords
Veterinary Public Health, Study