Allelic Variation for genes Associated with Erucic acid content in Indian Mustard (BRASSICA JUNCEA L. Czern & Coss)

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dc.contributor.authorGill, Karanjot Singh
dc.contributor.editorGurpreet Kaur
dc.date.accessioned2018-01-12T09:40:17Z
dc.date.available2018-01-12T09:40:17Z
dc.date.issued2017
dc.description.abstractPresent study was undertaken to investigate allelic variation for erucic acid content in B. juncea. 31 diverse germplasm lines of B. juncea (27), B. rapa (2) and B. nigra (2) representing different geographic origins (European, Australian and Indian) were characterized for allelic variation in relation to erucic acid. Erucic acid content of these germplasm lines ranged from 0 to 52.2 per cent. In diploids, single FAE 1.1 gene, whereas in amphiploids, two FAE1.1 and FAE1.2 genes exist. Complete sequence and coding sequence information of both genes FAE1.1 and FAE 1.2 from high as well as low erucic acid genotypes of B. rapa, B. nigra and B. juncea indicated that both genes were 1521 bp long and comprised only single exon. Sequencing data clearly revealed the role of point or mis-sense mutations for high/low erucic acid. Four SNPs for FAE 1.1 and three SNPs for FAE 1.2 were considered for characterizing high/low erucic acid genotypes. For FAE 1.1 gene, two types of markers, KASPar and CAPS marker were developed for SNPs at 735 and 968 position respectively, whereas for FAE 1.2 gene, KASPar markers were designed for SNPs at position 1476. These markers were validated on about 31 diverse germplasm lines. Further CAPS (FAE1.1) and KASPar (FAE1.2) were also validated on F2 population segregating for erucic acid content. Genotyping data on germplasm lines as well as F2 population by CAPS/KASPar markers revealed co-segregation with erucic acid content. CAPS and KASPar markers developed during present studies are highly efficient and can be used for marker assisted breeding for low erucic acid content.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810039286
dc.keywordsErucic acid, Fatty acid elongase gene, CAPS, KASPar, Brassica species, Mustarden_US
dc.language.isoenen_US
dc.pages68en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.subGenetics and Plant Breedingen_US
dc.subjectnullen_US
dc.titleAllelic Variation for genes Associated with Erucic acid content in Indian Mustard (BRASSICA JUNCEA L. Czern & Coss)en_US
dc.typeThesisen_US
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