Effect of Arsenic on common carp Cyprinus carpio (Linn.) And its Amelioration by Ascorbic acid
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Date
2019
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Punjab Agricultural University, Ludhiana
Abstract
Arsenic, a metalloid has become a potent toxicant in aquatic ecosystems because of wide
range of anthropogenic activities which has resulted in hazardous impact on aquatic fauna and
eventually humans who are a part of food chain.The present study aimed at examining the
toxicity of arsenic in Cyprinus carpio by conducting acute and chronic toxicity bioassays and
evaluating the ameliorating potential of ascorbic acid to combat arsenic toxicity. Acute
toxicity bioassay resulted in 96hr LC50 of 107.05 mg/l. Chronic toxicity bioassay was
conducted by exposing fishes to 1/0th and 1/5th LC50 of arsenic. Fishes were divided into six
experimental groups viz. Negative control, Positive control, 1/5thLC50As, 1/10thLC50As,
1/5thLC50As+AA and 1/10thLC50As+AA. A significant decrease in the TEC, Hb, Hct and a
significant increase in TLC was reported in 1/5th and 1/10thAs exposed groups when
compared with negative control and a significant improvement was observed in the blood
indices of As+AA treated groups. Antioxidant enzyme activity of SOD, CAT, GPx, GR and
GST was found to decline in response to arsenic exposure while an increment in their activity
was recorded in As+AA treated fishes. Lipid and protein content in the flesh of fish declined
significantly in 1/5th and 1/10th As treatment whereas the decline in ascorbic acid
supplemented groups was found to be significantly less in comparison to the respective
arsenic groups. Accumulation of arsenic was found to be significantly higher in 1/5th and
1/10th As treated groups. Uptake of arsenic was found to decrease on ascorbic acid
supplementation and depuration of arsenic was found to be 100% in 1/0th LC50 arsenic treated
groups. Histomorphologcal alteration observed in gills, liver and kidney were also found to be
less intense in As+AA than their respective arsenic treated groups. The results of present
study indicate that ascorbic acid is an effective ameliorating agent against deleterious effects
of arsenic in fish fauna and rehabilitation proves to be an effective method for complete
depuration of arsenic from fish flesh.
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