Functional study of genes involved in Maize seed and seedling development
| dc.contributor.advisor | Kumar, Rajeev | |
| dc.contributor.author | Ahmad, Mahtab | |
| dc.date.accessioned | 2020-02-04T06:58:47Z | |
| dc.date.available | 2020-02-04T06:58:47Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | There were several single gene recessive mutants analyzed in maize (Zea mays) such as lil1-1 homozygous (lil1-1/lil1-1/Nan1-1/Nan1-1) or heterozygous (lil1-1/lil1-1/Nan1-1/nan1-1), and another mutant was nan1-1 homozygous (nan1-1/nan1-1/Lil1-1/Lil1-1) or heterozygous (nan1-1/nan1-1/Lil1-1/lil1-1). The mutant lil1-1 was originally isolated from the selfed progeny of a Mutator stock outcrossed to an unrelated stock. The lil1-1 mutant allele was introgressed into B73, A188, H99, and Rsc-m2 inbred lines. The nan1-1 mutant was obtained from the Maize Genetics Cooperation Stock Centre. The mutant lilliputian (lil1-1), attributed to the insertion of a Mutator1 element in the first exon of the gene on chromosome number 2, encoding the BR C-6 oxidase the enzyme belongs to the superfamily of CYP85A proteins and catalyzes the final step of brassinosteroid biosynthesis. Due to the insertion of a transposable element, the lil1-1 mutant exhibits a reproducible phenotype consisting of a large primary root, extremely reduced stature and crinkly leaves. In Zea mays, another BR-deficient mutant was recently characterized, nan1-1 plant1 (na1), which is located on the long arm of chromosome number third, carry a loss-of-function mutation in a DET2 analog of Arabidopsis, a gene encoding a 5 α-steroid reductase and catalyzes the initial step of BR biosynthesis pathway. These mutant nan1-1 and lil1-1 genotypes were confirmed through four pairs of an allele or gene-specific primer. The lil1-1 mutant is expressed as much drought tolerance in comparison Lil1-1 wild type. We were analyzed the emb (embryo specific) mutants indicated that most frequently the embryo arrest in early embryogenesis at the proembryo, transition or coleoptilar stage. There were several mutants in maize, had been induced by chemical or transpositional mutagenesis, the two basic compartments of the seed which mainly affected by this mutagenesis were amenable for experimental analysis. These mutants were not complemented, exhibit small or incomplete developed Name of the student : Mahtab Ahmad Registration Number : M/AB/118/2015-16 Major Advisor : Dr. Rajeev Kumar The degree to be awarded : M.Sc. (Agril. Biotechnology) Department : Agricultural Biotechnology & Molecular Biology Major Subject : Agricultural Biotechnology Minor Subject : Plant Breeding and Genetics Year : 2019 Total pages of the Thesis : 1-51+i-vi (Bibliography) Title of the Thesis : “Functional Study of Genes Involved in Maize Seed and Seedling Development”. embryos and endosperms. Some mutant was less frequently, impaired in their endosperm development with normal embryos, generally defined as dek (defective kernel). The empty mutant pericarp (emp) represented that the class of emp-dek and emp-ref mutants with very much reduction in endosperm development. These mutants were easily identified in segregating mature ears because they had devoid of endosperm and flattened by compression. In the current research work, we have used inbred maize lines such as- B73, Mo17, A188, W22, A636, and W64A. The inbred line B73 could potentially be an excellent value for maize breeding through crosses with inbred line Mo17 or to its relatives to develop commercial hybrids and even through intercrossing among related B73 lines for trait recombination purposes to create a good initial breeding material for the derivation of new B73-type inbreds. The molecular markers are useful for helping to define heterotic groups and to examine the relationships among inbred lines at the DNA level. The inbred lines Mo17 was used as resistant and susceptible controls. The B73 and Mo17 inbred lines are an important source of germplasm for U.S. maize hybrids on behalf of the significant of heterotic response that occurs whenever these two inbreds are crossed. The interest of researchers in seed biology has led to a better knowledge of the mechanisms of seed development, which prerequisite for new biotechnological as well as conventional breeding procedures. The Genetic analysis of the maize reveals the investigation of the molecular mechanisms responsible for seed development and provides the opportunity to perform the dissection of complex developmental processes and identify the monogenic mutants and their molecular characteristics because seeds are very much an important source of food, feed, and other commercial purposes. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810142417 | |
| dc.keywords | Plant, Seed, Maize, Zea mays, Breeding, B73, Mo17, A188, W22, A636, W64A | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | DRPCAU, Pusa, Samastipur | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Functional study of genes involved in Maize seed and seedling development | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Functional study of genes involved in Maize seed and seedling development | en_US |
| dc.type | Thesis | en_US |