Propagation Studies on Clonal Rootstocks of Apple (Malus × domestica Borkh.)

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dc.contributor.advisorJamwal, Mahital
dc.contributor.authorLal, Manmohan
dc.date.accessioned2021-10-27T09:31:09Z
dc.date.available2021-10-27T09:31:09Z
dc.date.issued2021-09
dc.description.abstractThe present investigations entitled “Propagation studies on clonal rootstocks of apple (Malus × domestica Borkh.)” was carried out at Plant Tissue Culture Laboratory, School of Biotechnology (SBT) and Tissue Culture Laboratory, Advanced Centre for Horticulture Research (ACHR), Udheywalla of Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu to standardize the in vitro propagation protocol of clonal apple rootstocks MM106 and MM111 during 2018. The shoot tip and nodal segment used as explants were taken from MM106 and MM111 mother block maintained at Regional Horticulture Research Sub Station, Bhaderwah, SKUAST-Jammu. Results from the present investigation revealed that different treatments of mercuric chloride (HgCl2) and sodium hypochlorite (NaOCl) showed a significant effect on per cent aseptic and per cent survival of cultures. Treatment of 0.1 per cent HgCl2 for 4 minutes resulted in maximum aseptic and survival of cultures during all the months of study. MS medium supplemented with 10 mg/l polyvinyl pyrrolidone was found to be the best antioxidant medium for reducing phenolic exudation from the excised portion of the explants and for culture establishment of explants. Nodal segments of clonal apple rootstock MM106 and MM111 resulted in maximum percentage of culture establishment i.e. 32.22 per cent and 47.88 per cent, respectively as compared to shoot tip explants. MS medium supplemented with 1.0 mg/l BAP + 1.0 mg/l GA3 was found to be the best medium for culture establishment in minimum number of days. Nodal segments were found to be more suitable explants than shoot tips which resulted in maximum culture establishment percentage of 57.77 per cent and 76.66 per cent in 26.33 days and 25.40 days for rootstocks MM106 and MM111, respectively. However, different media combinations also showed a significant influence on in vitro shoot proliferation. Nodal segments of clonal apple rootstocks MM106 and MM111 were found to be better in comparison to shoot tip explants wherein minimum number of days taken for shoot proliferation were recorded as 46.26 and 44.70, highest number of shoots per explant as 4.76 and 5.43, length of the longest shoot as 3.42 cm and 3.47 cm and highest number of leaves per explant as 10.33 and 11.00, respectively on MS medium supplemented with 1.0 mg/l BAP + 1.0 mg/l GA3 along with 0.2 per cent NAA. Moreover, different media compositions showed a significant effect on in vitro rooting of clonal apple rootstocks. The clonal apple rootstock MM111 was found to be superior for in vitro rooting response as compared to MM106 rootstock. Half strength MS medium supplemented with 1.0 mg/l NAA along with 0.2 per cent activated charcoal resulted in maximum rooted cultures as 60.00 per cent and 66.67 per cent and took minimum number of days of root initiation as 14.00 and 13.00 in clonal apple rootstocks MM106 and MM111, respectively. Maximum number of roots per explant as 5.00 and 5.67 and maximum root length as 3.70 cm and 3.75 cm in clonal apple rootstock MM106 and MM111, respectively were also recorded with half strength MS medium supplemented with 1.0 mg/l NAA along with 0.2 per cent activated charcoal. The potting mixture containing soil: sand: vermiculite: FYM :: 1:1:1:1 was found to be the most appropriate for the growth of in vitro raised plantlets of clonal apple rootstocks MM106 and MM111 which resulted in maximum survival of 73.33 per cent and 76.67 per cent, plant height as 3.23 cm and 3.30 cm and number of leaves/ plantlets as 9.00 and 11.00, respectively. The unit cost of one in vitro raised plantlets of clonal apple rootstocks i.e. MM106 and MM111 in present investigation was estimated to be ` 44.56 and ` 36.95, respectively including both tissue culture production phase and greenhouse production phase.en_US
dc.identifier.citationPreferred for your work: Google citationen_US
dc.identifier.other36720
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810177426
dc.keywordsCLONAL ROOTSTOCK, PROPAGATION, IN VITRO, APPLE, ECONOMICS, SHOOT TIP, NODAL SEGMENTen_US
dc.language.isoEnglishen_US
dc.pages110en_US
dc.publisherSher-e-Kashmir University of Agricultural Sciences and Technology of Jammuen_US
dc.subFruit Scienceen_US
dc.themePropagation Studies on Clonal Rootstocks of Apple (Malus × domestica Borkh.)en_US
dc.these.typePh.Den_US
dc.titlePropagation Studies on Clonal Rootstocks of Apple (Malus × domestica Borkh.)en_US
dc.typeThesisen_US
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