EVALUATION OF THE UTERINE FLUID AND ENDOCRINE CHANGES ASSOCIATED WITH METOESTRUAL BLEEDING IN DAIRY CATTLE
Loading...
Date
2022-09-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE, WAYANAD, KERALA VETERINARY AND ANIMAL SCIENCES UNIVERSITY
Abstract
The present study was conducted with the objective to evaluate the changes in uterine fluid, serum oestrogen and progesterone profiles associated with metoestrual bleeding in dairy cattle. The study was conducted in Group I animals (n=12) which included cows with the historyof profuse metoestrual bleeding and Group II cows (n=12) without metoestrual bleeding. A total 24 cows were studied for the rheological properties of cervical mucus discharge (CMD), endometrial cytology and biochemical changes of uterine fluid during oestrus (day 0) and metoestrus (day 2). The serum progesterone and oestrogen concentrations on days 0, 2 and 10 of oestrous cycle were estimated by RIA. Per rectal examination revealed persistence of dominant follicle (58.33 per cent) on ovaries and high uterine tonicity during metoestrus in group I cows. Majority of group I cows exhibited high oestrus intensity and prolonged duration of oestrus. The Group I cows voided CMD with bloody opaque, thin/watery consistency and ‘nil’ fern pattern during metoestrus. While the Group II cows exhibited CMD with turbid, thick/viscous and atypical fern pattern during metoestrus. The mean ± SE pH of CMD of Group I cows (7.96±0.10) had significantly (p<0.01) higher values than that of cows group II cows (7.64±0.07) during metoestrus. The mean spinnbarkeit values of Group I cows (9.35±0.50 cm) was significantly (p<0.01) higher than that of Group II cows (7.75±0.30) during metoestrus. The mean serum progesterone concentration of Group I cows (1.61±0.55 ng/mL) was significantly (p<0.05) higher than that of Group II cows (0.36±0.07 ng/mL) on day of oestrus. The mean serum oestradiol concentration of Group I cows (6.85±0.53 pg/mL) was significantly (p<0.05) lower when compared to Group II cows (8.48±0.49 pg/mL). Cytology revealed the PMN cell per cent during metoestrus was significantly (p<0.01) higher in Group I cows (4.04±0.25 per cent) than in Group II cows (3.02±0.11 per cent). A significantly (p<0.01) higher LE activity of uterine fluid was observed in Group I cows (142.5±33.26 cells/μL) than that of Group II cows (7.5±2.26 cells/μL) during metoestrus. In conclusion, the oestrus characteristics, the pH and spinnbarkeit value of CMD, serum progesterone and oestrogen concentration, PMN cell per cent and LE activity of uterine fluid during metoestrual bleeding was significantly varied. The overall conception rate in these cows (33.33 per cent) was found lower than that of the cows without metoestrual bleeding (83.33 per cent)