Molecular characterization and identification of candidate markers for seed hardness in soybean (glycine max (L.) merr.)
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Date
2019-08-09
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
The present investigation was carried out at Vilasrao Deshmukh College of Agricultural Biotechnology, Latur (M.S.) with a view to characterize and validate the DNA markers linked to seed hardness traits at molecular-level in a given soybean germplasm during 2018-19. Efforts were made to study DNA polymorphism via SSR and ISSR markers linked to seed hardness in the given set of soybean accessions. The study revealed significant genotypic differences for the hardness related traits evaluated in RBD indicated considerable amount of variations among these accessions. These 36 soybean accessions were further grouped as soft, slightly hard and hard categories on the basis of seed hardness and also categorized as light, intermediate and heavy for test weight.
Significant genetic diversity was observed among the analyzed soybean accessions, revealed by both UPGMA and PCA analysis. The SSR analysis yielded 87.50% polymorphism with corresponding 411 amplicons, whereas ISSR analysis yielded 83.81% polymorphism with 961 amplicons. PIC value ranged from
0.05 (Satt547) to 0.99 (Satt341) for SSR with an average PIC value of 0.56 per primer. For ISSR, PIC value ranged from 0.11(UBC826) to 0.68 (ISSR09) with an average PIC value of 0.47 per primer. The genetic distances ranged from 0.31 to
0.50 with an average of 0.36 among these thirty-six soybean accessions. Thus, the most polymorphic and seed hardness-linked informative candidate markers namely, Satt341, Sat_392, Satt531, Satt153, Satt586 and Satt313, were identified and validated in the given germplasm of Glycine max towards presence of such positive QTLs/ loci. The elite accessions (i.e. Cat2047 and Cat1398) revealed significantly with high test weight and low seed hardness values can be directly used as donor parent(s) in the empirical soybean breeding program.
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