STUDIES ON PHYSICO-CHEMICAL AND MOLECULAR CHARACTERIZATION OF PUMMELO (Citrus grandis L.) GENOTYPES
| dc.contributor.advisor | Mukhim, C. | |
| dc.contributor.author | BANSAL, RITU | |
| dc.date.accessioned | 2023-02-04T11:16:13Z | |
| dc.date.available | 2023-02-04T11:16:13Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | The present experiment entitled “Studies on physico-chemical and molecular characterization of pummelo (Citrus grandis L.) genotypes” was conducted at RPCAU, Pusa, Samastipur, Bihar on pummelo during October 2021 to June 2022 to assess the variation in pummelo genotypes based on physico-biochemical characters and to characterize the pummelo genotypes using SSR markers. Diversity was analyzed by using principal component analysis and cluster analysis in thirty physico-biochemical characters of twenty-five genotypes. Physical characters of fruit, seed, peel and biochemical characters such as TSS, titrable acidity, ascorbic acid content, total sugar content, reducing and non-reducing sugar of the fruits were recorded. The highest fruit weight (1583.33 g) and fruit diameter (18.26 cm) were recorded in PC 5, however, maximum fruit length (15.96 cm) was observed in PC 14 whereas, minimum (9.03 cm) in PC 3. Different fruit shapes such as spheroid, obolid, ellipsoid and oblique were recorded in twenty-five genotypes. Shape of fruit base in pummelo genotypes were observed such as concave, truncate and convex. Most of genotypes were exhibited semi-solid and solid fruit axis with irregular cross section of fruit axis shape. Eighteen genotypes displayed low oil gland density. Red and pink color pulp were observed in most of genotypes while other genotypes exhibited cream, yellow, pinkish cream and pinkish yellow color pulp. Seed shape among different genotypes showed a lot of variation such as semi-deltoid, ovoid, spheroid and clavate. Six genotypes viz., PC 6, PC 7, PC 11, PC 12, PC 16 and PC 20 were found to be seedless. A significant variation in biochemical content was found among different genotypes. The total soluble solids, titrable acidity and ascorbic acid content were found in the range of 4.2-10 ºBrix, 0.43-1.04 per cent and 23.46- 47.77 mg/100ml juice respectively. The total sugar, reducing sugar and non-reducing sugar content varied from 5.66-9.28 %, 2.23-4.73 % and 2.65-4.56 %, respectively. Principal Component 1 alone exhibited 89.67 per cent of the total variation present among the 25 pummelo genotypes. First two Principal Components described more than 96 per cent variability in different genotypes. Among the primers, fifteen of them exhibited amplifications. The highest PIC value (0.87) was found in M-57 whereas, lowest (0) in M-86 and M-45. The genetic diversity based on SSR markers showed less to moderate diversity present in the genotypes which was explained by Nei ‘s genetic diversity index (h) and Shannon ‘s information index (I) with a mean h value of 0.32 and I value of 0.48. Four major clusters were obtained based on dice dissimilarity index from 15 SSR data matrix of twenty-five pummelo genotypes, where cluster I comprised two genotypes viz., PC 13 and PC 11, cluster II consisted two sub-clusters and comprised 9 genotypes, cluster III further divided into two sub-clusters which comprised 9 genotypes and cluster IV comprised 5 genotypes. The principal co-ordinates analysis map showed that genotypes like PC 2, PC 14, PC 15 and PC 7 are closely related or similar and are diverse from remaining genotypes. These twenty-five genotypes were admixture of four genetic structures which was analyzed using STRUCTURE HARVESTER. The present research finding can be used for future breeding and crop improvement programme. | en_US |
| dc.identifier.other | M/HORT. (FS)/494/2020-21 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810193280 | |
| dc.keywords | Physico-biochemical parameters, Genetic Diversity, Molecular Characterization, Principal Component Analysis, Cluster Analysis. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 92 + (i-xv) Bibliography + Appendix | en_US |
| dc.publisher | DRPCAU, PUSA | en_US |
| dc.sub | Fruit Science | en_US |
| dc.theme | Studies on physico-chemical and molecular characterization of pummelo (Citrus grandis L.) genotypes | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | STUDIES ON PHYSICO-CHEMICAL AND MOLECULAR CHARACTERIZATION OF PUMMELO (Citrus grandis L.) GENOTYPES | en_US |
| dc.type | Thesis | en_US |