MOLECULAR CHARACTERIZATION AND SEROMONITORING OF CANINE PARVOVIRUS FOLLOWING MODIFIED LIVE CPV-2b VACCINATION IN PUPS
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Date
2017
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
This study focused on the influence of maternally derived antibodies on the development of serum antibody titres among pups subsequent to vaccination with a commercially available multicomponent vaccine containing high-titre low-passage CPV strain. The pups were divided into two groups based upon the estimation of CPV antibody titres on day 0 by HI and ELISA. Estimation of the titres by HI test revealed that by day 15, 16 out of 23 pups from group One and 24 out of 27 pups from group Two had protective titres. All 50 pups attained protective titres by day 30 but only 33 (66 per cent) retained it by day 180. Results of the ELISA showed 19 out of 20 puppies in group One and 19 out of 30 in group Two were having protective titres on day 15. All but one of the 50 pups became protected by day 30, but only 37 including the pup that was non-protected on day 30 remained with protective serum titres. These results indicate that vaccination with a high-tire low-passage CPV strain can induce protective immunity by day 30 post vaccination irrespective of the MDA status of the vaccinates. Extracts of faecal DNA followed by PCR and sequencing coupled with comparison against existing sequences revealed presence of New CPV-2a in 19 samples while the other two were CPV-2a indicating that New CPV-2a was the prevailing strain in Thrissur district
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KVASU REFERENCE CITATION