Effect of exogenous phenolic acids on antioxidative defense system of wheat seedlings under salt stress
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Date
2016
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Punjab Agricultural University, Ludhiana
Abstract
In the present study, effect of exogenous phenolic acids on antioxidative defense system of
salt stressed wheat seedlings was investigated at 7th day of growth. Electrical conductivity of
10 dS/m was used for imposing salt stress. For observing stress mitigating effects of various
phenolic acids, 20 ppm of ferulic, 10 ppm of caffeic, 10 ppm of p-coumaric, 5 ppm of
salicylic and 15 ppm of sinapic acids were selected for biochemical studies. Catalase and
POX activities were upregulated in the stressed shoots of salt tolerant cultivar by the
exogenous use of caffeic and sinapic acids. In comparison to stress, APX activity was also
upregulated in stressed seedlings of both cultivars by exogenous use of caffeic and sinapic
acids. In stressed seedlings of salt sensitive cultivar, MDHAR activity increased by
exogenous use of caffeic, p-coumaric, salicylic and sinapic acids. In roots of Kharchia Local,
use of ferulic, para-coumaric and caffeic acids resulted into upregulation of GR activity,
whereas in salt sensitive cultivar only caffeic acid caused upregulation of this enzyme. Total
phenols also increased in salt stressed seedlings of both the cultivars on application of ferulic,
caffeic and salicylic acids which was partially attributed to the downregulation of PPO
activity. Proline content increased in HD 2329 by use of different exogenous phenolic acids
whereas, in Kharchia Local use of sinapic acid led to enhanced proline content. Glycine
betaine content was increased by use of different phenolic acids in the stressed roots of
Kharchia Local. On the other hand, exogenous application of sinapic acid led to enhanced
glycine betaine content in salt sensitive cultivar. In the shoots of both cultivars, MDA content
either decreased or remained unaffected whereas H2O2 decreased in both cultivars by
exogenous use of different phenolic acids.
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