Capri-Paratuberculosis: Prevalence and characterization by molecular diagnostic techniques

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dc.contributor.advisor. Upadhyay, S.R.
dc.contributor.authorNabi, Burhan
dc.date.accessioned2023-01-04T06:34:56Z
dc.date.available2023-01-04T06:34:56Z
dc.date.issued2022-08-03
dc.description.abstractThe present study was carried out with objectives to study the sero-prevalence of MAP in caprine population, to evaluate various diagnostic options for MAP andrepercussions of Johne’s disease on haemetobiochemical and oxidative stress parameters. The diagnosis of disease was done using multiple tests viz., faecal microscopy, indirect ELISA, faecal IS900 PCR, faecal IS1311 PCR-REA.The prevalence was determined by screening faecal smears of animals, stained by Ziehl-Neelsen stain, for acid fast bacilli (AFB) indistinguishable from MAP (Mycobacterium Avium subsp. Paratuberculosis) and measuring antibody titer in the serum of animals using indirect ELISA. The prevalence of AFB shedders was found to be 53%with highest in age group 1-2 yearsfollowed by 2-3 year and >3 years old age group. The seroprevalence of MAP in Jammu division was found to be 73.9 % with maximum in age group 1-2 yearfollowed by2-3 year and >3 years old age group. A total of 90 samples were put to IS900 PCR, and 15 samples showed amplification. These 15 samples were put to IS1311 PCR-REA (Restriction Endonuclease Analysis) and only 4 samples showed amplification product pattern similar to Type B (Bison) strain of MAP. The sensitivity and specificity of indirect tests (faecal microscopy, indirect ELISA) was calculated using faecal PCR as gold standard. The indirect ELISA was found to be more sensitive (76.9%) test for diagnosis of MAP. Animals positive for MAP had significant decrease in haemoglobin, PCV %, lymphocyte count, total protein, albumin, calcium and magnesium levels along with neutrophilia as compared to healthy animals.Oxidative stress was evident in JD affected animals from significant decrease in SOD, GPx, CATactivity when compared to animals negative for MAP.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810191136
dc.keywordsAFB,ELISA, IS900 PCR, IS1311 PCR-REA,JOHNE’S DISEASE, MAPen_US
dc.language.isoEnglishen_US
dc.pages124pen_US
dc.publisherSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu(UT of J&K)en_US
dc.research.problemCapri-Paratuberculosis: Prevalence and characterization by molecular diagnostic techniquesen_US
dc.subVeterinary Medicineen_US
dc.themeCapri-Paratuberculosis: Prevalence and characterization by molecular diagnostic techniquesen_US
dc.these.typePh.Den_US
dc.titleCapri-Paratuberculosis: Prevalence and characterization by molecular diagnostic techniquesen_US
dc.typeThesisen_US
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