Characterisation of Carrot (Daucus carota L.) genotypes Based on biochemical and molecular markers

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dc.contributor.advisorSarao, Navraj Kaur
dc.contributor.authorSandhu, Harsimerdeep Kaur
dc.date.accessioned2018-01-10T06:09:14Z
dc.date.available2018-01-10T06:09:14Z
dc.date.issued2017
dc.description.abstractThe characterization of 45 genotypes of carrot grown during 2015-16 season was done for morphological biochemical as well as molecular markers. Variability was observed for all morphological as well as biochemical traits however juice content, dry weight, carotene content, anthocyanin content and total soluble sugars were having wide variability among various genotypes. Genetic diversity and fingerprint profiles of these 45 Daucus genotypes were also determined using 55 microsatellite markers. Twenty five markers out of total amplified fifty one were polymorphic yielding a total of 85 alleles at 2921 loci, with an average of 1.55 alleles per marker. A unique DNA profile was generated by 11 markers yielding 19 unique alleles thus identified 18 genotypes. Genetic variability was assessed by diversity analysis using Darwin 6.0. All the genotypes were clustered into six major clusters irrespective of their geographic origin. The major differences among genotypes were identified by markers belonging to genome sequenced SSRs and Bac sequenced SSRs. Maximum number of alleles were amplified by Daucus carota L. specific marker DCM-2. Therefore, the biochemical traits as well as the SSR markers are effective tools to identify diversity or closeness among genotypes which may contribute to fasten the breeding programs and be fruitful for successful carrot crop improvement programs.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810039151
dc.keywordsCarrot, morphological, biochemical, molecular, SSRsen_US
dc.language.isoenen_US
dc.pages65en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemCharacterisation of Carrot (Daucus carota L.) genotypes Based on biochemical and molecular markersen_US
dc.subAgricultural Biotechnologyen_US
dc.subjectnullen_US
dc.themeCharacterisation of Carrot (Daucus carota L.) genotypes Based on biochemical and molecular markersen_US
dc.these.typeM.Scen_US
dc.titleCharacterisation of Carrot (Daucus carota L.) genotypes Based on biochemical and molecular markersen_US
dc.typeThesisen_US
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