EVALUATION OF GENOTOXICITY FOLLOWING REPEATED ORAL ADMINISTRATION OF CYPERMETHRIN IN WISTAR RATS

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dc.contributor.advisorThaker, A. M.
dc.contributor.authorMORE, AMAR SUNIL
dc.date.accessioned2018-05-25T05:38:27Z
dc.date.available2018-05-25T05:38:27Z
dc.date.issued2008
dc.description.abstractPyrethroids have become the most frequently used pesticides because of more stability in light and good insecticidal activity. Cypermethrin is an insecticide of the synthetic pyrethroid family. Over ninety percent of the cypermethrin, manufactured worldwide, is used to kill insects mainly on cotton and other agricultural crops. It is also used on lettuce and pecans to kill cockroaches (and other indoor pests), in buildings and to kill termites. As this insecticide is in use as a crop protectant, it is likely to cause indirect exposure of animals and humans through contaminated feed, soil and water, leading to some biological alterations. Hence present study was conducted to find out genotoxic potential of cypermethrin. The present study was conducted in 60 healthy male and female Wistar rats, which were acclimatized for one week before the start of oral dosing of cypermethrin. Approximate median lethal dose (ALD50) of cypermethrin used for the study was 250 mg/kg body weight. Sixty rats were divided into five different group, each comprising 12 rats. Each group had 6 male and 6 female rats .The groups were numbered as group I to V. The group I was given lower toxic dose of cypermethrin (1/40th part of LD 50 i.e. 6.25 mg/kg) orally by gavaging for 28 days, group II was given medium toxic dose of cypermethrin (1/30th part of LD 50 i.e. 8.33 mg/kg) orally by gavaging for 28 days, group III was given higher toxic dose of cypermethrin (1/20th part of LD 50 i.e. 12.33 mg/kg) orally by gavaging for 28 days, group IV was gavaged with com oil for 28 days and was kept as negative control and group V served as a positive control group and was given cyclophosphamide for genotoxicity study (20 mg/kg) intra peritoneally 24 hours prior to sacrifice. Once daily oral dosing of test compound was carried out for 28 days. All the rats were monitored for any observable toxic symptoms throughout the experimental period and they were also weighed weekly to monitor weight gain. Before sacrificing, the blood samples of rats were collected and were analyzed for percentage comet cells through comet assay test. After sacrification, bone marrow of femur was collected for both micronuclei and chromosomal aberration tests. Micronuclei test was performed on first half i.e. 30 rats and chromosomal aberration test was performed on rest half i.e. another 30 rats. There were noticeable signs and symptoms like pawing, burrowing and tremors. Severity of symptoms was high in animals given high dose of cypermethrin. During the last quarter of the experiment, animals in higher dose groups appeared dull, depressed and anorexic. Besides there was no mortality at all the dose levels of cypermethrin administration. There was no significant effect on body weight gain of the cypermethrin treated rats as compared to control. There was increase in polychromatic erythrocytes with micronuclei when dose of cypermethrin was increased and was significantly higher than vehicle controls at all dose levels. It was also observed that induction of MN in the rats treated with highest dose of cypermethrin was significantly low as compared to cyclophosphamide treated positive group.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810047505
dc.keywordsEVALUATION OF GENOTOXICITY, FOLLOWING REPEATED ORAL ADMINISTRATION, CYPERMETHRIN IN WISTAR RATSen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemEVALUATION OF GENOTOXICITY FOLLOWING REPEATED ORAL ADMINISTRATION OF CYPERMETHRIN IN WISTAR RATSen_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.subjectVETERINARY PHARMACOLOGY & TOXICOLOGYen_US
dc.subjectEVALUATIONen_US
dc.themeEVALUATION OF GENOTOXICITY FOLLOWING REPEATED ORAL ADMINISTRATION OF CYPERMETHRIN IN WISTAR RATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleEVALUATION OF GENOTOXICITY FOLLOWING REPEATED ORAL ADMINISTRATION OF CYPERMETHRIN IN WISTAR RATSen_US
dc.typeThesisen_US
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M.V.Sc. (Veterinary Pharmacology and Toxicology) Dissertation
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