STATUS OF ANTI-RABIES ANTIBODIES IN DOGS AND COMPARATIVE ANALYSIS OF RABIES VIRUS DETECTION TESTS
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Date
2015
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Publisher
AAU, Anand
Abstract
The present study was aimed to study rabies virus neutralising antibody status
in dogs by ELISA (PLATELIA™ RABIES II ASSAY kit, Bio-Rad) and also
comparative evaluation of various rabies virus detection tests. A total of 107 serum
samples were collected from vaccinated pet dogs at TVCC, College of Veterinary
science and Animal Husbandry, Anand and from Veterinary Poly Clinic, Vadodara
and 53 samples from unvaccinated stray dogs at Animal Birth Control Programme
(ABC) unit, Ahmedabad to detect rabies virus neutralizing antibodies. The antibody
titres obtained by quantitative ELISA were converted into EU/ml as per
manufacturer’s instruction by graphpad prism software. Of these, 58 (62.36%) pet
dogs and one out of 53 (1.88%) stray dogs showed antibody titre above the cut-off
value of 0.5 EU/ml. Vaccinated pet dogs were divided into different groups based on
age, sex, breed, vaccine brand and time of vaccination after last vaccine to assess the
relationship of these factors with vaccinal immune response. One way analysis of
variance (ANOVA) was performed in graphpad prism software to check the effect of
all these factors. Statistical analysis of ELISA titres of pet dog serum samples
suggested that age, sex, breed, vaccine brands have no significant effect on the antirabies
antibody titres. Fourteen non vaccinated pet dog serum samples were checked
to detect the maternal antibody status against rabies and of these, only two puppies
(aged 2.5 and 2 months) had a protective immune titre above the cut off value of 0.5
EU/ml.
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Keywords
veterinary science, study