COMPARATIVE EFFICACY OF DIAGNOSTIC TESTS FOR PARATUBERCULOSIS IN GOATS

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dc.contributor.advisorM.R. Saseendranath
dc.contributor.authorS. SULFICAR
dc.date.accessioned2020-03-13T07:08:00Z
dc.date.available2020-03-13T07:08:00Z
dc.date.issued2008
dc.description.abstractParatuberculosis (Johne's disease) is a chronic debilitating infection ot goats caused by Mycobacterium avium subsp paratuberculosis (MAP). Rece. t .} the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the present study comparative efficacy of acid fast staining of faecal smear, single intradermal Johnin test and IS900 faecal PGR were investigated in two hundred goats for detection of MAP. Single intradermal Johnin test was carried out in two hundred goats. Faecal sample from all two hundred goats subjected to Ziehl-Neelsen acid fast staimng and IS900 PGR for detection of MAP. Out of one hundred and fifty goats screened for paratuberculosis from University sheep and goat farm, Mannuthy. five animals (3.33 per cent) were found positive by acid fast staining, eight animals (5.3 per cent) were found positive by single intradermal Johnin test and thirty animals (20 per cent) were found positive by IS900 PGR. Out of fifty goats screened for paratuberculosis from field, one goau (2 per cent), three goats (6 per cent) and twelve animals (24 per cent) were found positive by Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS900 PGR respectively Results of present study from both farm and field indicate that amplification of the IS900 insertion element was the most specific and sensitive diagnostic method. The strategic use of PGR can provide a means for early identification of MAP infected goat, thus ensuring their elimination from an infected herd. Advantage of skin test is its ease of application and its low cost. Culling of CM! test positives could be a cost effective means of removing infected animals before they actually start faecal shedding. Ziehl-Neelsen staining had the lowest detection rate of the three tests under investigation and it proved unreliable in diagnosing Johne's disease.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810145353
dc.keywordsCOMPARATIVE EFFICACY DIAGNOSTIC TESTS PARATUBERCULOSIS GOATSen_US
dc.language.isoenen_US
dc.pages114en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSURen_US
dc.research.problemCOMPARATIVE EFFICACY OF DIAGNOSTIC TESTS FOR PARATUBERCULOSIS IN GOATSen_US
dc.subPreventive Veterinary Medicine and Epidemiologyen_US
dc.subjectnullen_US
dc.themeCOMPARATIVE EFFICACY OF DIAGNOSTIC TESTS FOR PARATUBERCULOSIS IN GOATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleCOMPARATIVE EFFICACY OF DIAGNOSTIC TESTS FOR PARATUBERCULOSIS IN GOATSen_US
dc.typeThesisen_US
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