Genotyping alien introgression lines of Brassica juncea (L.) Coss. and mapping of gene(s) for resistance to Sclerotinia sclerotiorum (Lib.) de Bary

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dc.contributor.advisorBanga, S.S.
dc.contributor.authorRana, Kusum
dc.date.accessioned2016-08-19T15:42:32Z
dc.date.available2016-08-19T15:42:32Z
dc.date.issued2016
dc.description.abstractTwo sets of Brassica juncea introgression lines (ILs) carrying introgressed chromosome fragments from two wild Brassicaceae species, Brassica fruticulosa and Erucastrum cardaminoides were investigated for their resistance responses to stem from inoculation with Sclerotinia sclerotiorum, the world wide pathogen of crop brassicas. Molecular characterization of ILs was also undertaken using SSR and candidate gene based markers. The percentage of donor genome in the B. juncea-B. fruticulosa ILs varied from 2.1 (IL-283) to 30.2% (IL-248). A total of 22 significant marker trait associations were detected across two crop seasons. Of these, 14 marker loci were A-genome specific; eight were B-genome specific. Five were common across the seasons. Significantly associated markers were SB4817, SJ4933, SJ3640I, SJ0644, SB1871, nia-m043, nia-m138, cnu-m482, cnu-m298, cnu-m245 and cnu-m254. These introgression lines were also genotyped using 60 gene-specific markers developed from the sequence information of the candidate genes associated with resistance to Sclerotinia stem rot. Five significant marker trait associations (MTAs) were identified. Out of these, MTA involving ABI2-1 was consistent across the seasons and the pooled data. The phenotypic variance explained ranged from 21-30 percent. MTAs involving ABI1-3_1, also steady over seasons, accounted for 14 to 26 percent of the phenotypic variation. A second set of 96 B. juncea- E. cardaminoides introgression lines (ILs) were also genotyped with SSR markers and phenotyped for their resistance to Sclerotinia stem rot as well. Association mapping allowed the identification of six marker loci (based on A genome markers) influencing the resistance to Sclerotinia stem rot resistance. Of these, three were detected in both seasons as well as pooled data. In case of B genome based markers, six associations were observed in season I, 5 in S II and 5 in pooled over both seasons. These studies not only provided important new insights into the introgressed resistance but also opens the way for novel engineering of new B. juncea varieties that express this key resistance to this worldwide devastating pathogen of mustard cropsen_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/73069
dc.language.isoenen_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.subGenetics and Plant Breeding
dc.subjectAlien introgression, B. fruticulosa, Erucastrum cardaminoides, marker trait associations, Brassica juncea, Sclerotinia sclerotiorumen_US
dc.these.typePh.D
dc.titleGenotyping alien introgression lines of Brassica juncea (L.) Coss. and mapping of gene(s) for resistance to Sclerotinia sclerotiorum (Lib.) de Baryen_US
dc.typeThesisen_US
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