Synthesis, characterization and antimicrobial activity of microcapsules of carrot seed essential oil

Simple item page
dc.contributor.advisorUrvashi
dc.contributor.authorAmrinder Singh
dc.date.accessioned2023-01-08T05:28:33Z
dc.date.available2023-01-08T05:28:33Z
dc.date.issued2022
dc.description.abstractThe present investigation deals with chemistry, microencapsulation and antimicrobial potential of carrot (Daucus carota) seed essential oil. Carrot seeds were powered and subjected to hydrodistillation using Clevenger apparatus to extract essential oil. Acid value, saponification value and moisture content of the oil were 4.7 mg g-1, 153.6 mg g-1 and 8.0 %, respectively. Gas chromatography-mass spectrometry of carrot seed essential oil (CSEO) analysis showed the presence of 45 compounds. Major compounds of CSEO were carotol, daucol and daucene. Microcapsules of CSEO were prepared by using coacervation technique. Four combination ratios (Gelatin: Sodium alginate: CSEO) were prepared by varying the concentrations of gelatin and CSEO and keeping the concentration of sodium alginate constant. Unencapsulated and encapsulated CSEO was evaluated for its antimicrobial potential against two bacteria (Gram positive and Gram negative) Staphylococcus aureus and Escherichia coli using disk diffusion method. The inhibition zones of unencapsulated CSEO were found to be 18.0 mm and 13.0 against S. aureus and E. coli, respectively (Table 4.4). However, for encapsulated carrot essential oil the inhibition zones were found to be 15.0 mm and 11.6 mm against S. aureus and E. coli, respectively. Minimum inhibitory concentration (MIC) was also determined at various concentrations (0.1-100 mg ml-1). MIC value of CSEO was observed at 1.5 mg ml−1 while, the encapsulated CSEO displayed MIC value of 2.5 mg ml−1 against S. aureus. Both encapsulated and unencapsulated CSEO showed inhibition at 10 mg ml−1 against E. coli. Unencapsulated essential oil was more effective against both the tested bacteria as compared to encapsulated CSEO. However, encapsulated CSEO retained inhibition for longer durations.en_US
dc.identifier.citationAmrinder Singh (2022) Synthesis, characterization and antimicrobial activity of microcapsules of carrot seed essential oil (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810191506
dc.keywordsCarrot seed essential oil, microencapsulation, coacervation, antimicrobial studiesen_US
dc.language.isoEnglishen_US
dc.pages71en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemSynthesis, characterization and antimicrobial activity of microcapsules of carrot seed essential oilen_US
dc.subChemistryen_US
dc.themeSynthesis, characterization and antimicrobial activity of microcapsules of carrot seed essential oilen_US
dc.these.typeM.Scen_US
dc.titleSynthesis, characterization and antimicrobial activity of microcapsules of carrot seed essential oilen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Amrinder thesis after viva 24.1.2022.pdf
Size:
2.08 MB
Format:
Adobe Portable Document Format
Description:
M.Sc.
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis