Ante mortem diagnosis of co-infection of bovine tuberculosis and paratuberculosis
Loading...
Files
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
The aim of this study was to diagnose co-infection of bovine tuberculosis (bTB) and paratuberculosis (JD) in live animals using ante-mortem techniques such as comparative intradermal tuberculin test (CITT) and bovine gamma interferon assay (IFN-γ) for bTB and Single intradermal test (SID) for JD. Further molecular diagnosis of Mycobacterium tuberculosis complex (MTC) and M. avium subspecies paratuberculosis (MAP) by multiplex polymerase chain reaction (mPCR) was carried out using in house designed primers. Three hundred animals (94 cattle and 188 buffaloes) were selected from an organized dairy farm, while 18 cattle were from an unorganized dairy farm. The animals of age group between 2-12 years were included in the study. Blood and faecal samples were collected from the animals to be tested by CITT, IFN-γ, SID, conventional IS6110 PCR, IS900PCR and mPCR. Twenty one and nine animals were positive for bTB by CITT and IFN-γ assay respectively, out of which only nine animals were positive by both CITT and IFN-γ. Further by SID, nine animals were tested positive for JD. Four animals were positive reactors for both CITT and SID. All the blood samples were processed for conventional IS6110 PCR, IS900 and in-house mPCR. In IS6110 PCR 23 samples positive for MTC while four blood samples and five faecal samples were detected positive for MAP by IS900 PCR. A mPCR was developed to differentiate MAP and MTC in blood samples of cattle and buffaloes by observing a band size of 210 bp and 263 bp respectively. By in-house mPCR, one animal was positive result for both MTC and MAP. This animal was also positive for CITT/SID and IFN-γ. Thus, the in-house multiplex PCR may be useful to diagnose co-infection of bTB and paratuberculosis simultaneously.
Description
T1797