Effect of Trichoderma spp. in response to application of nanoparticles for management of Rhizoctonia disease of Black Gram
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Date
2023
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Abstract
An experiment was conducted to test the compatibility of nanoparticles with three different Trichoderma spp. namely Trichoderma viridae, Trichoderma harzianum, Trichoderma pseudokoningii at five different concentrations 200 ppm,400 ppm,600 ppm,800 ppm and 1000 ppm. Results revealed that out of three Trichoderma spp, T.harzianum was found to be compatible with ZnO NPs @ 200ppm where we observed maximum mycelail growth inhibition of 10.25% . Web blight caused by Rhizoctonia sp. was isolated from infected black gram plant which was collected from Instructional Cum Research Farm , AAU, Jorhat. Cultural, morphological and molecular identification revealed that the isolated was found to be Rhizoctonia solani. In vitro efficacy of , T. harzianum compatible with ZnO NPs showed mycelia growth inhibition of 73.91%.However highest inhibition percentage of 88.47% was observed in chemical treatment hexzconazole @0.1% In vivo assessment was made to find out the effect of T. harzianum supplemented with ZnO NPs @ 200ppm on disease incidence, severity, plant growth parameters, yield and yield attributing characters. Disease incidence was found to be in the range of (76.37 to 90.25 per cent) and disease severity was observed in the range of 87.53 to 95.12 .Highest plant growth parameters was like shoot length (35.79 Cm), root length (20.90Cm), fresh shoot weight(44.78g), fresh root weight (1.78g) , dry weight of shoot (4.30g) and dry weight of root (0.66g) was observed when soil and foliar application of T. harzianum supplemented with ZnO NPs @ 200ppm was done as compared to control. Highest yield and yield attributing characters like number of branches per plant (15.67), number of pods per plant (25.77), pod length (7.18 cm), yield per plant (9.59q/ha ) , and weight of 100 seed (4.38g) observed when soil and foliar application of T. harzianum supplemented with ZnO NPs @ 200ppm was done as compared to control. From present study, it can be concluded that the severity of web blight of black gram can significantly reduced along with increase in yield by the use of bioagent T. harzianum supplemented with ZnO NPs @ 200ppm by soil and foliar application @ 5gm/plant and 5ml/litr.