Immunodiagnosis of canine dirofilariosis using microfilarial antigen

dc.contributor.advisorSubramanian, H
dc.contributor.authorPriya, M N
dc.contributor.authorKAU
dc.date.accessioned2020-11-11T05:57:32Z
dc.date.available2020-11-11T05:57:32Z
dc.date.issued2003
dc.descriptionMVScen_US
dc.description.abstractA detailed study was conducted on the prevalence on canine dirofilariosis in Thrissur from June 2002 to May 2003, feasibility of microfilarial antibody detection by ELISA and comparison of sensitivity of Indirect ELISA and dot- ELISA in the detection of dirofilariosis. Microfilariae encountered were identified as those of Dirofilaria repens. Out of the total number of2435 blood samples from dogs screened by wet film examination 172 (7.06 per cent) dogs were found positive for microfilariae. It has been found that canine dirofilariosis was prevalent throughout the year with more incidence during summer. An Indirect ELISA and dot-ELISA were also conducted in three groups of animals using microfilarial antigen prepared from known microfilaria positive serum samples. Out of the total number of 50 samples of known microfilaria positive dogs 49 (98 per cent) and 47 (94 per cent) were found positive by Indirect ELISA and dot-ELISA respectively. The corresponding figures in 36 dogs with other helminths were 15 (41.67 per cent) and 19 (52.77 per cent) and that of32 totally helminth free dogs were 10 (31.25 per cent) and 12 (37.5 per cent) respectively. The results indicate that both Indirect ELISA and dot-ELISA are feasible for the detection of the microfilarial antibodies though they are less specific.en_US
dc.identifier.citation172232en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810154896
dc.keywordsVeterinary Parasitologyen_US
dc.language.isoEnglishen_US
dc.publisherDepartment of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthyen_US
dc.subVeterinary Parasitologyen_US
dc.themeCanine dirofilariosis using microfilarial antigenen_US
dc.these.typeM.V.Sc.en_US
dc.titleImmunodiagnosis of canine dirofilariosis using microfilarial antigenen_US
dc.typeThesisen_US
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