ANTIFUNGAL RESISTANCE OF DERMATOPHYTES ISOLATED FROM DOGS
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Date
2021-03-30
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR
Abstract
A total of 100 dogs were screened for dermatophytosis from December
2020 to September 2021 from Teaching Veterinary Clinical Complex (TVCC),
Mannuthy and University Veterinary Hospital (UVH), Kokkalai. Of total samples,
25 samples (25 per cent) yielded a positive culture for dermatophytosis on SDA
(Sabourauds Dextrose Agar). Five species of dermatophytes including T.
mentagrophytes, T. rubrum, M. gypseum M. nanum and E. floccosum were isolated
in the present study. Among them T. mentagrophyte (44 per cent) had higher
occurrence followed by T. rubrum (24 per cent), M. gypseum (20 per cent) M.
nanum (eight per cent) and E. floccosum (four per cent). Dermatophytosis was
most commonly seen in puppies of less than six months of age (48 per cent) than
young (36 per cent) and adult dogs (16 percent). Pure breeds of dog (92 per cent)
were more infected with dermatophytosis than non-descriptive breeds (8 per cent).
There was no significance difference noticed in sex wise predisposition of
dermatophytosis. Seventy-six per cent dermatophytosis cases were presented as
localized lesions and 24 per cent were presented as generalized lesions in the
present study. Occurrence of dermatophytosis was high during summer and
monsoon season than winter. Molecular characterization of dermatophyte isolates
was carried out by using three sets of primers (ITS1-ITS4, B2F-B4R and TR1-
TR2). Antifungal sensitivity was carried out by broth microdilution technique on
25 isolates with 4 antifungal agents (fluconazole, ketoconazole, miconazole and
griseofulvin). Four isolates of T. mentagrophytes, four isolates of T. rubrum and
three isolates of M. gypseum had high MICs for fluconazole (64 µg/ml), while
ketoconazole, miconazole and griseofulvin had the lowest MIC values for all the
isolates. So, it could be concluded that ketoconazole, miconazole and griseofulvin
are the more preferable antifungals to treat dermatophytosis than fluconazole.
Description
Thesis Submitted in the partial fulfillment of the requirement for the degree of Master of Veterinary Science in Veterinary Epidemiology and Preventive Medicine