MICROBIAL DEGRADATION OF MIMOSINE IN GOATS
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Date
1995
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College of Veterinary and animal Science,Mannuthy
Abstract
An investigation was carried out to find out the extent
of in vitro microbial degradation of pure mimosine (Tl) and
that of immature leaves (T2), mature leaves {T3), tender
stems (T4) and seeds (T5) of leucocephala using
strained rumen liquor obtained from three rumen fistulated
Saanen-Malabari crossbred goats maintained under standard
conditions of feeding and management. The proximate
chemical composition and mimosine content of different
edible parts of leucaena during the months of May, June and
July were determined.
While immature leaves and seeds had higher crude
protein content,seeds had higher crude fat, tender stems had
higher crude fibre and mature leaves had higher ash content
compared to other edible parts of subabul.
The average mimosine concentrations of T2, T3, T4
and T5 were 12.11±0.05, 4.89±0.02, 3.90±0.04 and 10.70±0.08
per cent respectively during May; 11.66±0.06, 5.23±0.03,
3.62±0.03 and 10.44±0.05 per cent respectively during June
and 9.96±0.05, 4.92±0.03, 3.73±0.02 and 9.51±0.04 per cent
respectively during July on a dry matter basis.
The average mimosine concentrations of strained goat
rumen liquor incubated with 37.50 mg/100 ml of added
mimosine in pure form or as immature leaves, mature leaves,
tender stems and seeds showed significant reduction at
every 12 hr intervals from 0 to 48 hr of incubation, the
final average concentrations being 23.98 ±0.37, 23.14±0.37,
22.20±0.28, 23.12±0.52, 23.35±0.37 mg/100 ml of SRL.
The percentages of in vitro degradation in respect of
Tl, T2, T3, T4 and T5 increased significantly at every 12 hr
intervals of incubation from 0 to 48 hr, even though the
degradation was incomplete with all treatments, the average
percentage degradation at 48 hr of incubation being
31.69±1.02, 34.49±1.18, 37.12±0.99, 34.54±1.50 and
33.41+1.03 respectively.
The overall average rate of disappearance of mimosine
in |ig.ml~^.h~^ in respect of Tl, T2, T3, T4 and T5 for the
entire period of 48 hr of incubation were 2.33, 2.54, 2.74,
2.54 and 2.44 respectively with highest rates during 0 to 12
hr, lower rates during 24 to 36 hr and least rates during 36
to 48 hr.
The production of ammonia and VFA coincided with the
active degradation of mimosine, there being faster
degradation upto 12 hr of incubation with highest
concentrations of ammonia and VFA at 12 hr of incubation.
The overall results indicated that the rumen
microorganisms of crossbred goats degrade mimosine to DPH,
ammonia and VFA and that mimosine does not inhibit the
microbial activity, even though the possible role of
leucaena endogenous enzymes in the partial degradation of
mimosine recorded in the present study cannot be ruled out.
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