SEROEPIDEMIOLOGY AND ELECTROPHEROGRAM STUDIES OF BLUETONGUE VIRUS

Simple item page
dc.contributor.advisorJhala, M. K.
dc.contributor.authorBhalodiya, M. B.
dc.date.accessioned2018-07-02T12:05:25Z
dc.date.available2018-07-02T12:05:25Z
dc.date.issued2000
dc.description.abstractBluetongue (BT) is an insect transmitted viral disease of several species of domestic and wild ruminants. The disease is a cause for serious concern to livestock industry due to staggering direct and indirect economic losses. In many countries like India having considerable sheep population, the disease has become endemic. Severity of the infection depends upon the species and breed of animals, serotypes/strains of virus and prevalent ecosystem. The present study was aimed to find out prevalence of Bluetongue virus (BTV) antibodies in cattle and sheep of different regions of the Gujarat State. Avidin-Biotin enzyme-linked immunosorbent assay (AB-ELISA) and indirect enzyme-linked immunosorbent assay (I-ELISA) employed for antibody detection were also compared in terms of their sensitivity and specificity. An attempt was also made to standardize polyacrylamide gel electrophoresis (PAGE) for detecting BTV. Out of 527 cattle and sheep sera tested, 343 (65.08%) and 396 (75.14%) were found to be positive for BTV antibodies by AB-ELISA and IELISA respectively. Specieswise, 69.04 and 77.57 percent of cattle and 55.55 and 63.33 percent of sheep revealed antibodies to BTV by AB-ELISA and l-ELISA respectively. The highest prevalence rate was found in South Gujarat region (67.94 and 78.63 percent) by AB-ELISA and l-ELISA respectively and female animals (65.53 and 77.84 percent) showed more prevalence than male animals (64.35 and 70.79 percent). Districtwise seroprevalence study revealed highest incidence in Valasad district (68.51%), followed by Navsari (67.70%), Kutch (60.74%), Junagadh (57.69%) and Rajkot (51.72%) district by AB-ELISA, whereas IELISA yielded highest prevalence rate in Valasad (80.55%) followed by Navsari (77.82%), Rajkot (69.96%), Junagadh (69.23%)) and Kutch (66.96%>) district. In cattle, highest incidence was observed in Kankrej (75.52%)) by ABELISA, while HF cross-yielded highest positive samples (84.72%) by l-ELISA. Lowest incidence was seen in Gir breed by both the tests (51.51 and 66.66 percent), while in sheep, higher prevalence rate in native Patanwadi breed (64.28 and 73.21 percent) was observed than the crossbred (41.17 and 47.05 percent) by AB-ELISA and I-ELISA respectively. Comparison of l-ELISA and AB-ELISA for the detection of BTV antibodies, revealed that the former test was better in terms of sensitivity and specificity. Out of a total 527 serum samples tested 343 (65.08%) and (75.14%) reacted positively in AB-ELISA and l-ELISA respectively. l-ELISA detected BTV antibodies in 54 (10.25%) samples, which were negative by AB-ELISA. Relative sensitivity and specificity of AB-ELISA to l-ELISA were 86.63 and 99.23% respectively and overall agreement between both the tests was 89.56%. RNA-PAGE was employed for detecting BTV using a cell culture propagated BTV serotype 1. This was attempted essentially to standardize this highly sensitive technique, so as to use if routinely in future for the field samples. The study revealed 10 RNA segments with a migration pattern resembling to serotype 1 of BTV.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810056477
dc.keywordsSEROEPIDEMIOLOGY, ELECTROPHEROGRAM STUDIES, BLUETONGUE VIRUSen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemSEROEPIDEMIOLOGY AND ELECTROPHEROGRAM STUDIES OF BLUETONGUE VIRUSen_US
dc.subVeterinary Microbiologyen_US
dc.subjectVETERINARY MICROBIOLOGYen_US
dc.subjectA STUDYen_US
dc.themeSEROEPIDEMIOLOGY AND ELECTROPHEROGRAM STUDIES OF BLUETONGUE VIRUSen_US
dc.these.typeM.V.Sc.en_US
dc.titleSEROEPIDEMIOLOGY AND ELECTROPHEROGRAM STUDIES OF BLUETONGUE VIRUSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
M. B. Bhalodiya.pdf
Size:
4.07 MB
Format:
Adobe Portable Document Format
Description:
M.V.Sc. (Veterinary Microbiology) Dissertation
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses