DETECTION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS IN ASIAN ELEPHANTS (ELEPHAS MAXIMUS)

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dc.contributor.advisorShyma V. H
dc.contributor.authorPUNYA M S
dc.date.accessioned2023-02-23T10:44:54Z
dc.date.available2023-02-23T10:44:54Z
dc.date.issued2021-08-30
dc.descriptionSubmitted in partial fulfillment of the requirement for the degree of Master of Veterinary Science in Veterinary Epidemiology and Preventive Medicine.en_US
dc.description.abstractElephant endotheliotropic herpesvirus (EEHV) has emerged as a major threat to the survival of endangered Asian elephant population. Elephant endotheliotropic herpesvirus (EEHV) infection in Asian elephants is an acute fatal haemorrhagic disease primarily affecting juvenile Asian elephants. The present study was proposed to confirm the presence of EEHV infection in Asian elephants and identification of the chronically infected shedders which will help in controlling the spread of disease among elephant population. A total of 31 elephants presented to Teaching Veterinary Clinical Complex, Mannuthy, and those under private ownership (Thrissur districts), temples and Department of Forests and Wildlife, Kerala from Thiruvananthapuram, Pathanamthitta and Ernakulam during 2019- 2020 were included in this study. The animals were subjected to physical examination and assessment of haemato-biochemical parameters. The faecal examination revealed strongyle ova in 22.7 per cent cases and strongyloides ova and larva in 9.7 per cent cases. Trunk wash samples were collected for PCR. Out of 31 samples, one yielded positive for EEHV and EEHV1 genotype. Sequencing of the positive PCR product confirmed amplicon similarity to EEHV1. The remaining 30 elephants failed to give the amplicons for EEHV. Haemato-biochemical studies revealed leukopenia, lymphocytosis and hypoalbuminemia in the affected animal. Post mortem samples of two elephants from Nilambur forest were subjected to PCR and not found to have the amplicons of EEHV.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810194345
dc.keywordsAsian elephants, Elephant endotheliotrophic herpesvirus , Aetiology , Nucleic acid detection assays, Serological assays , Acute phase protein expression, Viral microRNA detection,en_US
dc.language.isoEnglishen_US
dc.pages102p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, MANNUTHY, THRISSURen_US
dc.research.problemDetection of elephant endotheliotropic herpesvirus (EEHV) infection in Asian elephants by polymerase chain reaction (PCR) techniqueen_US
dc.subPreventive Veterinary Medicine and Epidemiologyen_US
dc.themeDETECTION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS IN ASIAN ELEPHANTS (ELEPHAS MAXIMUS)en_US
dc.these.typeM.V.Sc.en_US
dc.titleDETECTION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS IN ASIAN ELEPHANTS (ELEPHAS MAXIMUS)en_US
dc.typeThesisen_US
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