Pyramiding bacterial blight resistance genes (Xa21, Xa38) in maintainer and cytoplasmic male sterile line of rice (Oryza sativa L.) through marker assisted selection

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dc.contributor.advisorThind, K.S.
dc.contributor.authorLego, Nancy
dc.date.accessioned2019-11-11T04:41:49Z
dc.date.available2019-11-11T04:41:49Z
dc.date.issued2019
dc.description.abstractHybrid rice has great potential in certain districts of Punjab. However, most of the hybrids are susceptible to bacterial blight (BB). In the present study BB resistance genes (Xa21 and Xa38) have been pyramided through marker assisted selection (MAS) into PCMS 16A/B which has good outcrossing ability. An elite breeding line, PAU 5942-1-1-1-1-1-1 with good agronomic and quality characters and possessing Xa21 and Xa38 genes was used as donor parent. Marker assisted foreground selection was carried out using the gene specific markers pTA248 for Xa21 and Os04g53050-1 for Xa38 and background selection was done using 64 polymorphic SSR markers between donor and recipient parent. The recipient parent genome (RPG) recovery ranged from 80.5 to 92.9% in BC2F1 plants. A set of 254 BC2F2 progenies carrying different combinations of Xa21/Xa38 genes along with parents were screened for BB resistance using pathotype X of BB prevalent in Punjab. Based on the RPG% and BB screening, 11 BC2F2 plants were found to be desirable. Agronomically superior lines of improved PCMS 16B line are being converted into CMS line through backcrossing. The improved BB resistant CMS lines can be further used in developing hybrid varieties resistant against all the 10 pathotypes of BB in Punjab. Further, a set of 109 testcross hybrids were generated using 79 diverse rice genotypes and 21 Wild abortive (WA) based-CMS lines to identify new maintainers and restorers from the pool of locally adapted germplasm for the development of component lines in a hybrid breeding program. Molecular markers DRRM-RF3-10 and DRCG-RF4-14 were used to screen for the presence of restorer genes Rf-3 and Rf-4 in these 79 genotypes. Among these genotypes, 56% carried Rf4Rf4/rf3rf3, 10% carried Rf3Rf3/rf4rf4, 10% carried Rf3Rf3/Rf4Rf4 and 24% carried rf3rf3/rf4rf4 allelic combination. Rf-4 was found to be predominant than Rf-3. Based of spikelet fertility 17 maintainers, 17 partial maintainers, 18 partial restorers and 37 restorers were identified. The molecular and testcross results revealed differential fertility behaviour of genotypes with different WA based-CMS lines.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810134999
dc.keywordsBacterial blight, MAS, Xa21, Xa38, maintainers, restorers, CMSen_US
dc.language.isoenen_US
dc.pages80en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemPyramiding bacterial blight resistance genes (Xa21, Xa38) in maintainer and cytoplasmic male sterile line of rice (Oryza sativa L.) through marker assisted selectionen_US
dc.subGenetics and Plant Breedingen_US
dc.subjectnullen_US
dc.themePyramiding bacterial blight resistance genes (Xa21, Xa38) in maintainer and cytoplasmic male sterile line of rice (Oryza sativa L.) through marker assisted selectionen_US
dc.these.typePh.Den_US
dc.titlePyramiding bacterial blight resistance genes (Xa21, Xa38) in maintainer and cytoplasmic male sterile line of rice (Oryza sativa L.) through marker assisted selectionen_US
dc.typeThesisen_US
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