ENDOMETRIAL CYTOLOGY IN POSTPARTUM CROSS-BRED DAIRY COWS AND ITS CORRELATION WITH FERTILITY
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Date
2016
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College of Veterinary and animal Science,Mannuthy
Abstract
The study was undertaken with the objectives of characterizing
endometrial cytology in postpartum dairy cows and determining the prevalence of
subclinical endometritis with reference to its effect on subsequent fertility. The
study was conducted in KVASU farms at Mannuthy and Thumboormuzhi.
Twenty-four apparently healthy postpartum cross-bred dairy cows were subjected
to detailed clinico-gynaecological and vaginoscopic examination. Endometrial
sampling was done using uterine cytobrush (UC) and uterine lavage (UL) on 30,
40 and 50 days postpartum (DPP). The mean (±SE) per cent of polymorphonuclear (PMN) cells by UC were 7.53 ± 1.56, 3.23 ± 1.75 and 1.35 ± 2.21 in normal group and 9.80 ± 1.84, 10.20 ± 2.07 and 17.01 ± 2.61 in
endometritis group on 30, 40 and 50 DPP, respectively. Whereas by UL, the PMN
cells per cent were 7.64 ± 1.76, 4.92 ± 1.41 and 1.21 ± 1.74 in normal group and 11.20 ± 2.08, 14.60 ± 1.67 and 21.70 ± 2.06 in endometritis group, respectively,
on 30, 40 and 50 DPP. In normal group, significant difference between UC and
UL on both 30 and 40 DPP. In the endometritis group, significant difference was
observed between UC and UL on 30, 40 and 50 DPP. The endometritis group
cows were treated with intrauterine infusion of 100 µg E.coli lipopolysaccharide
(LPS). The mean (±SE) pH of vaginal discharge in normal and endometritis group
was 7.17 ± 0.15 and 8.20 ± 0.11, respectively and pre and post intrauterine
treatment of endometritis group was 8.20 ± 0.11 and 7.40 ± 0.12, respectively.
The conception rates after first, second and third service (overall) observed in this
study were 42.86, 64.29 and 78.57 per cent in normal group; 20, 50 and 60 per
cent, respectively, in endometritis group after LPS treatment. From the results of
the study, it could be inferred that endometrial cytology could be used for a
presumptive diagnosis of SCE. Metricheck and disposable vaginal speculum are
recommended for routine diagnosis of endometritis. The prevalance of
endometritis in KVASU farms was found to be 41.67 per cent. E.coli LPS has a
reasonable therapeutic effect in the management of SCE and thereby increase the
conception rate.
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KVASU reference style