Characterization Of Escherichia Coli Isolates From The Cases Of Calf Diarrhoea Using Polymerase Chain Reaction
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Date
2004
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
The present study was undertaken to isolate Escherichia coli from calf diarrhoea cases and to study the role of different virulence factors in the pathogenesis of the disease. A total of 50 faecal samples from buffalo and cow calves showing signs of diarrhoea were collected from five organized dairy farms of Punjab and a detailed study for isolation, identification, serotyping, biotyping, antibiogram pattern and characterization of different virulence genes of E. coli isolates was carried out. E. coli were isolated from all the samples and were differentiated into 42 biotypes on the basis of biochemical tests. Out of 50 E. coli isolates 4 were rough, 1 was Maximum isolates belonged to serogroup 0101 (7) followed by 04 (5); 0127 (4); 09, 055, 073 (3 each); 01, 033, 0107, 0132 (2 each) and rest of the 12 serogroups were having a single isolate. Antibiogram pattern was conducted by using 30 different antibiotics, which revealed that fiirazolidone, clindamycin and cephadroxil were 100 per cent sensitive for all the isolates. A multiplex PCR based assay was carried out targeting 6 different virulence genes viz. stxl, stx2, eaeA, F41, K99 and Sta, based on which 50 isolates were classified into three categories i.e. STEC (VTEC) with 36 (72%) isolates, ETEC 10 (20%) and EPEC 2 (4%) isolates. It was observed that stx/ was the most common virulent gene in STEC (VTEC) followed by eaeA and there was a strong association between stx/ and eaeA