Phenotypic Characterization and Molecular Profiling of Sweet and Non-sweet Corn Genotypes and their F1s for Soluble Sugar Content in the stem
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Date
2015-09-28
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Corn is among the top five cereals in the world and third most important food
crop in India. The sweet corn is also becoming increasingly popular in India and other
Asian countries. Sweetcorn altogether with non-sweet corn produces huge volume of
biomass residue that will be an alternative raw material for biofuel production. In this
study both phenotypic and molecular level diversity were analyzed with respect to
soluble sugar content in the stem among sweet (15) and non-sweet (7) corn parents and
their hybrids (66) during Kharif 2014. Sweetcorn hybrid- Madhuri used as check.
Analysis of variance revealed that there was significant variation for all the traits studied
at both 10 and 20 days post silk emergence. Traits which account for soluble sugars in the
stem showed significant positive correlation with plant height, number of internodes,
plant weight, stem weight, stem girth, juice volume and juice extraction percentage.
Sweet × non-sweet crosses were found to have highest positive significant mid-parent
heterosis for stem sugars indicating that cytoplasm of the sweet corn had significant
effect on these traits. Ten PCR primers specific to the ZmSUT1 gene responsible for
sucrose transport in corn were designed using NCBI database. Out of these, ZmSUT1b,
ZmSUT1c and ZmSUT1f were found to be polymorphic, indicating the presence of
genotypic variation among them for the trait of interest. It is suggested that the biomass
of sweetcorn along with hybrids developed using sweetcorn as female parent will be
suitable resources as biofuel feedstock
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