DNA Fingerprinting of brinjal (Solanum melongena L) varieties and related species
| dc.contributor.advisor | Nazeem, P A | |
| dc.contributor.author | Vikhe, Parimal Laxman | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2018-11-15T11:56:16Z | |
| dc.date.available | 2018-11-15T11:56:16Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Brinjal (Solanum melongena L.) also known as aubergine or eggplant, is a member of the family Solanaceae. It is an important vegetable in central, southern and south-east Asia, and in a number of African countries. In India, it is one of the most common, popular and principal vegetable crop grown throughout the country except in higher altitudes. The study entitled “DNA fingerprinting of brinjal (Solanum melongena L.) variet ies and related species” was carried out at the Center for Plant Biotechnology and Molecular Biology, College of Horticulture Vellanikkara during the period 20112013. The objective of study was to characterize the four brinjal varieties released by Kerala Agricultural University using molecular markers like ISSR and SSR and to develop a DNA fingerprint specific to each variety. Three superior accessions and two wild relatives were also characterized to detect the level of variability. The brinjal varieties/accessions selected include three Solanum melongena varieties- Surya, Swetha, Haritha and the hybrid Neelima; three accessions- SM 116, SM 396, SM 397 and the two wild relatives S. melongena var. insanum and S. macrocarpon. Breeder seeds were obtained from the Department of Olericulture, College of Horticulture, Kerala Agricultural University and maintained at CPBMB, COH and further used for the study. Morphological characters were recorded for six vegetative and five reproductive characters according to the descriptor of NBPGR. The data recorded was used to validate the genotypes which was used for fingerprinting. DNA extraction was done according to the method described by Rogers and Bendich 1994. The RNA contamination was completely removed through RNase treatment. Good quality DNA with UV absorbance ratio (OD 260 /OD ) 1.80 – 1.89 was used for further analysis. 280 Thirty six ISSR primers and sixty one SSR primer pairs were screened with DNA of brinjal genotypes for amplification and those which gave reliable distinct banding pattern were selected for further amplification/fingerprinting. The PCR product obtained from ISSR and SSR analysis were separated on 2 per cent agarose gel and amplification patterns recorded. The genomic DNA from each genotype was amplified with 10 each of selected ISSR and SSR primers. The amplification pattern was scored and depicted to develop fingerprint for each brinjal genotype. The Resolving power (Rp) of the ISSR primers was calculated and the values ranged between 9.9 and 28.44 indicating the capacity of the primers selected to distinguish the genotype. The Polymorphic Information Content (PIC) was also calculated and it ranged between 0.83 and 0.96 for ISSR primers, further indicating the suitability of primers to detect polymorphism. The PIC value for SSR primers were zero and not suitable to detect polymorphism. Distinct amplicons developed through ISSR and SSR analysis were used to develop the DNA fingerprint of brinjal genotypes. Sharing of amplicons for each primer with other genotypes was also analyzed and demarcated with different colour codes in the fingerprint developed. Most of the amplicons were found shared among the genotypes indicating their genetic uniformity. However, the pattern of sharing was different and good enough to separate out the varieties and distinct amplicons were observed in the genotypes. The ISSR and SSR banding pattern was scored and analysed for their uniformity/variability using the software NTSYS pc (version 2.02i). Similarity coefficient ranged from 0.51 to 0.84. The highest similarity (84%) was observed between the brinjal hybrid Neelima and its maternal parent Surya. The S. macrocarpon was the most distinct one from other brinjal genotypes with 49 per cent variability. Separate fingerprint were developed for all the four varieties, three accessions and two wild relatives through ISSR and SSR analysis. The DNA fingerprints thus developed could be utilised for the variety registration and settling IPR issues. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810083497 | |
| dc.keywords | Plant Biotechnology and Molecular Biology | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | DNA Fingerprinting | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | DNA Fingerprinting of brinjal (Solanum melongena L) varieties and related species | en_US |
| dc.type | Thesis | en_US |