AUGMENTING FERTILITY IN ANOESTRUS AND REPEAT BREEDING BUFFALOES USING CONTROLLED BREEDING TECHNIQUES
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Date
2013
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AAU, Anand
Abstract
This study was carried out at 5 villages of Anand district under the AMUL milk shed area on 50 buffaloes, comprising 20 acyclic-true anoestrus,. 20 cyclic-repeat breeders and 10 normal cyclic buffaloes (exhibiting oestrus within 90 days postpartum). The objectives were to evaluate clinical response and monitor peripheral plasma progesterone, biochemical and macro-micro minerals profile at different time intervals in anoestrus (CIDR and Ovsynch protocol) and repeat breeding (Al+GnRH and Mid-cycle PGFaa inj.) buffaloes treated with different hormonal preparations. The effect of these protocols was evaluated by comparing oestrus induction response, oestrus induction interval and induced/first cycle (with fixed time AI) as well as overall of three cycles conception rates, and monitoring plasma progesterone by RIA, total cholesterol, total protein, calcium, inorganic phosphorus and magnesium by assay kits on auto-analyzer, and micro-minerals (Zn, Fe, Cu,. Co, Mn) profile using wet digested samples on atomic absorption spectrophotometer at different time intervals of treatment in anoestrus (day 0, 7, 9/10-Al) and in repeat breeding (day 0 and day of oestrus/Al) as well as normal cyclic control buffaloes and on day 21 post-Al in all the buffaloes. Ten true anoestrus buffaloes were inserted with i/vaginal CIDR (containing 1.38 g progesterone in silastic coil) for 7 days, it was removed on day 7 together with i/m Inj. of PGF2α 25 mg (Inj. Lutalyse, 5 ml) and FTAl was done on day 9 with i/m Inj. of GnRH 10 μg (Inj. Receptal, 2.5 ml). All the 10 (100 %) buffaloes exhibited induced ovulatory oestrus within stipulated time with moderate to prominent oestrus signs. The conception rates obtained at induced/first, second, third cycle and overall were 40.00 (4/10), 50.00 (3/6), 00.00 (0/3) and 70.00 (7/10) per cent, respectively. The interval from PGF2α injection to induced oestrus was 63.60 ± 6.46 hrs (n=10) and the fertile oestrus interval was 10.25 ± 3.94 days (n=7) among CIDR treated conceived buffaloes.
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Keywords
ANIMAL REPRODUCTION, GYNAECOLOGY AND OBSTETRICS, A STUDY