Discovery of DNA markers associated with root traits in Ragi [Eleusine coracana (L.) Gaertn] through phenotypic characterization and selective genotyping of a RIL population
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Date
2019-08-17
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK, BENGALURU
Abstract
Ragi is an important millet grown and consumed predominantly in the arid and
semiarid regions of the world. Since major area of ragi cultivation is under rainfed
conditions its yield is low, increasing its production by improving drought adoptive traits
such as root is important. However, screening for these traits is cumbersome. Therefore,
our current research was focused on development of high throughput phenotyping
methods for measuring root traits. Two genotypes were grown on clerigel, and coirpith to
select a best root phenotyping medium. Among these, genotypes grown on the coirpith
gave the good result, in which, average root length of parent 1 was 30 cm on 25th day,
while the other parent reached only 22.3 cm.
A mapping population comprising of 206 Recombinant inbreed lines were
screened for roots by growing them in root structures. At booting stage root traits were
measured by dismantling the structure. Root length of the high root parent was 45.29 cm,
while it was 36.29 cm for low parent. Root biomass of the RIL population varied from
1.25 g pl-1 to 6.35 g pl-1 with a mean of 3.36 g pl-1. Bulk segregant analysis was adopted
to identify the markers associated with root traits. Among the 102 SSRs polymorphic
between the parental lines, three markers UASBFM 85, UASBFM 91 and UASBFM 109
clearly showed specific root related segregation pattern in bulked segregant analysis.
These SSR markers can be considered as useful markers for genotyping root traits in ragi.