Biochemical and proteomic analysis of botrytis grey mould infected susceptible and resistant chickpea lines

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dc.contributor.advisorSharma, Sucheta
dc.contributor.authorThakur, Richa
dc.date.accessioned2019-04-22T13:13:42Z
dc.date.available2019-04-22T13:13:42Z
dc.date.issued2019
dc.description.abstractPresent investigation reports the morphological, ultrastructural and biochemical analysis of botrytis grey mould infected susceptible (PBG5) and resistant (C. pinnatifidum) chickpea genotypes. Seedlings of both the genotypes were inoculated with pregrown culture of B. cinerea (1 x 104 spores mL-1), using growth chamber and cut twig techniques. Inoculated leaves from both the genotypes were collected at 1, 3, 5 and 7 days post inoculation and used for further analysis. Visual disease symptoms were observed only in susceptible genotype PBG5 in terms of conidial colonies and water soaked lesions on leaves and stems. Scanning electron microscopic analysis revealed differences in cell surface topography between two genotypes with more number of stomata in PBG5 which were further validated by stomatal frequency analysis. Fourier transform infrared spectra of proteins were almost similar for both the genotypes. B. cinerea inoculation resulted in the induction of biochemical constituents viz. total phenols, malondialdehyde content and activities of CAT, APX, SOD, POX, PPO and PAL in both the genotypes with higher activities in resistant genotype than susceptible one at different days post inoculation. Isoenzymic analysis revealed the presence of one isoform of catalase, two in superoxide dismutase & three each in guaiacol peroxidase and polyphenol oxidase at 1 to 7 days postinoculation. SDS-PAGE analysis revealed absence of polypeptide bands with Rf (0.5, 0.21, 0.49 and 0.64) in PBG5 and (0.44, 0.60, 0.64 and 0.34) in C. pinnatifidum leaves at 3-7 days post B. cinerea inoculation in comparison to respective controls. At 5 and 7 days postinoculation, bands of molecular weight (97.4 and 66 kDa) were missing in the C. pinnatifidum leaves. Uninoculated chickpea genotypes, screened for botrytis responsive genes using PCR approach, indicated the presence of DNA band (~300 bp) in resistant genotype (C. pinnatifidum) suggesting its possible role for resistance against B. cinerea.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810100806
dc.keywordsBotrytis grey mould, chickpea, biochemical constituents, scanning electron microscopyen_US
dc.language.isoenen_US
dc.pages103en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemBiochemical and proteomic analysis of botrytis grey mould infected susceptible and resistant chickpea linesen_US
dc.subBiochemistryen_US
dc.subjectnullen_US
dc.themeBiochemical and proteomic analysis of botrytis grey mould infected susceptible and resistant chickpea linesen_US
dc.these.typePh.Den_US
dc.titleBiochemical and proteomic analysis of botrytis grey mould infected susceptible and resistant chickpea linesen_US
dc.typeThesisen_US
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