Genetic diversity analysis in dual purpose oat (Avena sativa L.) germplasm.
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
The present study was undertaken to assess the genetic diversity present in the ninety six oat (Avena sativa L.) germplasm lines representing the collection from various eco-geographical regions of the country. On the basis of mean performance of the genotypes for fodder traits; OL 10 for plant height (60.09cm), OL 1636 for leaf length (53.55cm) and JO 03-95 for leaf width (1.86 cm), OS 7 for GFY (3.38kg/plot) and JHO-2001-1 for DMY (0.61 kg/plot) were found to be superior. Similarly, on basis of mean performance of the genotypes for grain traits; OS 7 for beta-glucan (4.35%), JHO-2009-1 for grain length (15 mm), OL 1542, OL 1611, OL 1615, OL 1636, OL 1635, JHO 851, EC 605839, EC 605833, EC 209750, EC 209472, EC 209408, SKO 315, SKO 312, and RO-2001-1 for grain width (3.3 mm) and UPO-093 for grain yield (426.91 g/plot) were found to be superior. Genetic divergence among 96 accessions was worked out for fodder and grain traits and then for dual purpose to generate dendrogram based on complete linkage and squared euclidean distance. All the 96 accessions were grouped into 6 clusters. Maximum inter cluster distance for fodder, grain and dual purpose was recorded between clusters VI and III (9.99), clusters I and VI (9.06), clusters IV and V (11.24) respectively, suggesting significant high genetic diversity betweeen genotypes of these clusters. According to criteria followed by Proceedings of AICRP (FCU 2015), the 14 best dual purpose genotypes evaluated are: UPO 093, OL 1611, JHO-2001-1, HJ 114, JHO 851, OL 1635, OS 329, SKO 27, HJ 8, OS 363, OL 1714, OS 376, EC 605833 and JHO-2009-1.The molecular diversity analysis using 31 SSR markers clustered all the 96 germplasm lines into two clusters, cluster A and B. Cluster A was further divided into sub-cluster 1 and 2. Genotypic pairs having utmost genetic dissimilarity of 49 % were OL 1624; OS 6, EC 605839; SKO 312 and HJ 114; SKO 312 while the minimum genetic dissimilarity of about 8% was observed between the lines EC 209402 and OS 374. PIC values ranged from 0.08 to 0.82 (primer 1950-2) with an average of 0.47. A total of 100 alleles were detected by 31 primers in the 96 genotypes with an average of 3.22 alleles per primer. Total number of alleles amplified for each primer ranged from 2 - 8. The maximum number of alleles (8) was amplified by cAM19. In addition, statistically non significant correlation with r=0.195 (P=0.05) was reported between morpho- agronomic and molecular diversity.
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