Biochemical and molecular studies on different chickpea genotypes against infestation of pod borer, Helicoverpaarmigera (Hübner)
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Date
2018
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Dr. Rajendra Prasad Central Agricultural University, Pusa (Samastipur)
Abstract
During the course of investigation, Helicoverpa armigera was observed on chickpea genotypes from 51 to 114 days after sowing. The overall lowest mean larval population was recorded on Pusa 391 closely followed by RSG 888 during crop season 2017-18. The overall highest mean larval population (4.46) was recorded on JGK 1 which was at par with GJG 3, JAKI 9218, JG 315, JG 63 and JG 218.The maximum per cent pod damage (15.52%) was observed on JGK 1 genotype and minimum per cent pod damage (2.77%) in Pusa 391.
Results of biochemical experiments indicated that genotype JGK 1 recorded the lowest phenol (0.045 mg/g), flavonoids (0.047 mg/g) and tannin content (0.675 mg/g) and highest protein content (17.27g/100g) as compared to other genotypes. In genotype KAK 2 protease inhibitor activity was very high(18.22 mg/g), where as it was lowest (7.42%) in GJG 3. The genotypes JGK 1, GJG 3 and JG 315 with high protein content recorded higherper cent pod damage(15.52%, 13.25% and 12.44%, respectively) indicating that the genotypes were more preferred by the H. armigera. On the other hand, low per cent pod damage on Pusa 391 (2.77%) and Vishal (4.74%)may be attributed to low protein content.Highly significant and negative correlation was observed between pod damage (%) and all major biochemical parameters studied.
.On the basis of molecular studies, all the fifteen chickpea genotypes were divided into seven clusters. Eachcluster contains chickpea genotypes with similar
characteristics. Cluster A comprised of JG 11, cluster B consisted of ICCV 2 and KAK 2, cluster C consisted of Pusa 391 and RSG 888, cluster D consisted of JGK 1, cluster E contains GJG 3, JG 315, JG 218 and JG 63, cluster F contains ICCV 10, RSG 44 and Vishal, and the cluster G consisted of JAKI 9218 and KPG 59 genotype.
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